Applicability of crAssphage, pepper mild mottle virus, and tobacco mosaic virus as indicators of reduction of enteric viruses during wastewater treatment
Applicability of crAssphage, pepper mild mottle virus, and tobacco mosaic virus as indicators of reduction of enteric viruses during wastewater treatment
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DOI：
10.1038/s41598-020-60547-9
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发表时间：
2020-02-27
影响因子：
4.6
通讯作者：
Haramoto, Eiji
中科院分区：
文献类型：
--
作者：
Tandukar, Sarmila;Sherchan, Samendra P.;Haramoto, Eiji
本研究旨在评价CrAssphage、辣椒轻度斑驳病毒(PMMoV)和烟草花叶病毒(TMV)在废水处理过程中作为减少人类肠道病毒感染的指标的适用性。在13个月的时间里，从污水处理厂的三个步骤(未经处理的污水、二级处理的污水和最终出水)每月收集39个样本。除了这三种指示病毒外，还对8种人类肠道病毒[人腺病毒、JC和BK多瘤病毒、爱知病毒1号(AIV-1)、肠道病毒和I、II、IV基因群的诺如病毒]进行了定量PCR检测。除了少量crAssphage和TMV的最终流出物外，在被测试的样品中始终检测到指示性病毒。CrAssphage的平均浓度显著高于大多数测试病毒的浓度。原始污水中的crAssphage浓度与所有检测的人类肠道病毒浓度呈正相关(p
This study was conducted to evaluate the applicability of crAssphage, pepper mild mottle virus (PMMoV), and tobacco mosaic virus (TMV) as indicators of the reduction of human enteric viruses during wastewater treatment. Thirty-nine samples were collected from three steps at a wastewater treatment plant (raw sewage, secondary-treated sewage, and final effluent) monthly for a 13-month period. In addition to the three indicator viruses, eight human enteric viruses [human adenoviruses, JC and BK polyomaviruses, Aichi virus 1 (AiV-1), enteroviruses, and noroviruses of genogroups I, II, and IV] were tested by quantitative PCR. Indicator viruses were consistently detected in the tested samples, except for a few final effluents for crAssphage and TMV. The mean concentrations of crAssphage were significantly higher than those of most tested viruses. The concentrations of crAssphage in raw sewage were positively correlated with the concentrations of all tested human enteric viruses (p