A multifunctional teal-fluorescent Rosa26 reporter mouse line for Cre- and Flp-mediated recombination
A multifunctional teal-fluorescent Rosa26 reporter mouse line for Cre- and Flp-mediated recombination
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DOI：
10.1016/j.neures.2012.02.003
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发表时间：
2012-05-01
影响因子：
2.9
通讯作者：
Kageyama, Ryoichiro
中科院分区：
文献类型：
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作者：
Imayoshi, Itaru;Hirano, Kyoko;Kageyama, Ryoichiro
Cre 和/或 Flp 活性的报告基因对于定义 Cre/Flp 介导的重组的空间和时间范围非常重要。在这里，我们描述了 R26-CAG-LF-mTFP1，一种多功能荧光报告小鼠，在 Cre 和 Flp 介导的重组后强烈表达 mTFP1（明亮的青色荧光蛋白）。为了满足单一重组酶介导的报告基因表达的需要，我们生成了 R26-CAG-LF-mTFP1 的衍生物。 R26-CAG-LF-mTFP1 中 Frt 侧翼终止盒的种系切除产生了 Cre 依赖性报告基因（R26-CAG-LoxP-mTFP1）。类似地，R26-CAG-FRT-mTFP1（其中 loxP 侧翼的终止盒在种系中被切除）仅需要 Flp 来激活 mTFP1 表达。 (c) 2012 Elsevier Ireland Ltd 和日本神经科学学会。版权所有。
Reporters of Cre and/or Flp activity are important for defining the spatial and temporal extent of Cre/Flp-mediated recombination. Here, we describe R26-CAG-LF-mTFP1, a multifunctional fluorescent reporter mouse that strongly expresses mTFP1 (bright teal fluorescent protein) after Cre- and Flp-mediated recombination. To meet the need for single recombinase-mediated reporter expression, we generated derivatives of R26-CAG-LF-mTFP1. The germline excision of the Frt-flanked stop cassette in R26-CAG-LF-mTFP1 generated a Cre-dependent reporter (R26-CAG-LoxP-mTFP1). Similarly, R26-CAG-FRT-mTFP1, in which the loxP-flanked stop cassette was excised in the germline, requires only Flp to activate mTFP1 expression. (c) 2012 Elsevier Ireland Ltd and the Japan Neuroscience Society. All rights reserved.