Calcium oscillations in neocortical astrocytes under epileptiform conditions.
Calcium oscillations in neocortical astrocytes under epileptiform conditions.
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DOI：
10.1002/neu.10019
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发表时间：
2002
期刊：
影响因子：
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通讯作者：
A. Tashiro;J. Goldberg;R. Yuste
中科院分区：
文献类型：
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作者：
A. Tashiro;J. Goldberg;R. Yuste
星形胶质细胞的形态和功能的改变经常被发现在癫痫综合征，虽然星形胶质细胞在癫痫的确切作用知之甚少。在幼年新皮层切片癫痫样事件的钙成像过程中，我们先前发现细胞内游离钙浓度（[Ca（2+）]（i））具有自发振荡。我们现在已经使用两种体外癫痫模型表征了这些振荡，并发现它们是由星形胶质细胞产生的。星形细胞振荡在整个成像区域中广泛存在，非常有规律，周期长，平均为100 s，在发育过程中变得更短。星形胶质细胞振荡是不相关的，它们之间和癫痫样事件，被内部释放拮抗剂阻断，并受到咖啡因的刺激。星形胶质细胞钙振荡可能介导反应性星形胶质细胞增生，参与慢性癫痫综合征的发病机制，并可作为癫痫组织的诊断试验。
Morphological and functional alterations in astrocytic glia are often found in epileptic syndromes, although the exact role of astrocytes in epilepsy is poorly understood. During calcium imaging of epileptiform events in juvenile neocortical slices we previously discovered cells with spontaneous oscillations in their intracellular free calcium concentration ([Ca(2+)](i)). We have now characterized these oscillations using two in vitro models of epilepsy and find that they are produced by astrocytes. Astrocytic oscillations are widespread throughout the imaged territories, are remarkably regular and have long periods, averaging 100 s, which become shorter during development. Astrocytic oscillations are uncorrelated among themselves and with epileptiform events, are blocked by internal release antagonists and are stimulated by caffeine. Astrocytic calcium oscillations could mediate reactive astrogliosis, contribute to the pathogenesis of chronic epileptic syndromes, and be used as a diagnostic test for epileptic tissue.