Curcumin-induced apoptosis in scleroderma lung fibroblasts: role of protein kinase cepsilon.
Curcumin-induced apoptosis in scleroderma lung fibroblasts: role of protein kinase cepsilon.
复制标题
姜黄素诱导的硬皮病肺成纤维细胞凋亡：蛋白激酶ε的作用。
DOI：
10.1165/rcmb.2003-0354oc
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发表时间：
2004
影响因子：
6.4
通讯作者：
Hoffman,Stanley
中科院分区：
文献类型：
--
作者：
Tourkina,Elena;Gooz,Pal;Oates,JamesC;Ludwicka-Bradley,Anna;Silver,RichardM;Hoffman,Stanley
Scleroderma, a disease involving excessive collagen deposition, can be studied using fibroblasts cultured from affected tissues. We find that curcumin, the active component of the spice turmeric, causes apoptosis in scleroderma lung fibroblasts (SLF), but not in normal lung fibroblasts (NLF). This effect is likely to be linked to the fact that although curcumin induces the expression of the phase 2 detoxification enzymes heme oxygenase 1 and glutathione S-transferase P1 (GST P1) in NLF, SLF are deficient in these enzymes, particularly after curcumin treatment. The sensitivity of cells to curcumin-induced apoptosis and the expression of GST P1 (but not heme oxygenase 1) are regulated by the ε isoform of protein kinase C (PKCε). SLF, which contain less PKCε and less GST P1 than NLF, become less sensitive to curcumin-induced apoptosis and express higher levels of GST P1 when transfected with wild-type PKCε, but not with dominant-negative PKCε. Conversely, NLF become sensitive to curcumin-induced apoptosis and express lower levels of GST P1 when PKCε expression or function is inhibited. The subcellular distribution of PKCε also differs in NLF and SLF. PKCε is predominantly nuclear or perinuclear in NLF but is associated with stress fibers in SLF. Just as PKCε levels are lower in SLF than in NLFin vitro, PKCε expression is decreased in fibrotic lung tissuein vivo. In summary, our results suggest that a signaling pathway involving PKCε and phase 2 detoxification enzymes provides protection against curcumin-induced apoptosis in NLF and is defective in SLF. These observations suggest that curcumin may have therapeutic value in treating scleroderma, just as it has already been shown to protect rats from lung fibrosis induced by a variety of agents.
