Three-dimensional lithographically defined organotypic tissue arrays for quantitative analysis of morphogenesis and neoplastic progression
Three-dimensional lithographically defined organotypic tissue arrays for quantitative analysis of morphogenesis and neoplastic progression
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DOI：
10.1038/nprot.2008.35
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发表时间：
2008-01-01
期刊：
影响因子：
14.8
通讯作者：
Bissell, Mina J.
中科院分区：
文献类型：
--
作者：
Nelson, Celeste M.;Inman, Jamie L.;Bissell, Mina J.
在这里，我们描述了一个简单的微成型方法来构建三维阵列的器官型上皮组织结构，在体内组织学近似。包含具有确定几何形状和间距的柱阵列的弹性体印记用于在I型胶原蛋白凝胶的表面中模制微型空腔。将上皮细胞接种到腔中并覆盖第二层胶原蛋白。细胞重组成与腔的几何形状相对应的中空组织。图案化的组织阵列可以在3-4小时内产生，并将在随后的1-3天内经历形态发生。该协议可以很容易地适应于研究各种组织和正常和肿瘤发展的方面。
Here, we describe a simple micromolding method to construct three-dimensional arrays of organotypic epithelial tissue structures that approximate in vivo histology. An elastomeric stamp containing an array of posts of defined geometry and spacing is used to mold microscale cavities into the surface of type I collagen gels. Epithelial cells are seeded into the cavities and covered with a second layer of collagen. The cells reorganize into hollow tissues corresponding to the geometry of the cavities. Patterned tissue arrays can be produced in 3-4 h and will undergo morphogenesis over the following 1-3 d. The protocol can easily be adapted to study a variety of tissues and aspects of normal and neoplastic development.