COLOCALIZATION OF CALCIUM-DEPENDENT PROTEASE-II AND ONE OF ITS SUBSTRATES AT SITES OF CELL-ADHESION
COLOCALIZATION OF CALCIUM-DEPENDENT PROTEASE-II AND ONE OF ITS SUBSTRATES AT SITES OF CELL-ADHESION
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DOI：
10.1016/0092-8674(87)90126-7
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发表时间：
1987-11-20
期刊：
影响因子：
64.5
通讯作者：
CROALL, DE
中科院分区：
文献类型：
--
作者：
BECKERLE, MC;BURRIDGE, K;CROALL, DE
粘附斑是细胞与其基质接触的质膜的专门区域，是动态结构。然而，很少有人知道如何发生在粘附斑块的蛋白质-蛋白质相互作用的控制。细胞可能调节其与基质的结合的一种机制是通过粘附斑组分的选择性、受调节的蛋白水解。在这里，我们表明，催化亚基的钙依赖性蛋白酶I型（CDP-II）定位于粘附斑块的几种细胞类型（BS-C-1，EBTr，和MDBK）。我们比较了粘着斑成分粘着斑蛋白、talin和α-粘附素的敏感性。辅肌动蛋白的钙依赖性蛋白水解在体外，并已发现塔林是CDP-II的首选底物。需要钙的蛋白水解酶和塔林蛋白在粘附斑中的共定位提高了钙依赖性蛋白水解活性提供了通过塔林蛋白的裂解来调节粘附斑生理和功能的某些方面的机制的可能性。
Adhesion plaques, specialized regions of the plasma membrane where a cell contacts its substratum, are dynamic structures. However, little is known about how the protein-protein interactions that occur at adhesion plaques are controlled. One mechanism by which a cell might modulate its associations with the substratum is by selective, regulated proteolysis of an adhesion plaque component. Here we show that the catalytic subunit of the calcium-dependent protease type I (CDP-II) is localized in adhesion plaques of several cell types (BS-C-1, EBTr, and MDBK). We have compared the susceptibility of the adhesion plaque constituents vinculin, talin, and .alpha.-actinin to calcium-dependent proteolysis in vitro and have found talin to be the preferred substrate for CDP-II. The colocalization of a calcium-requiring proteolytic enzyme and talin in adhesion plaques raises the possibility that calcium-dependent proteolytic activity provides a mechanism for regulating some aspect of adhesion plaque physiology and function via cleavage of talin.