Role of Nonreceptor Protein Tyrosine Kinases During Phospholipase C-γ1-related Uterine Contractions in the Rat
Role of Nonreceptor Protein Tyrosine Kinases During Phospholipase C-γ1-related Uterine Contractions in the Rat
复制标题
非受体蛋白酪氨酸激酶在大鼠磷脂酶 C-γ1 相关子宫收缩中的作用
作者：
M. Phillippe;L. Sweet;Diana F. Bradley;D. Engle
激活的磷脂酶C1，产生于酪氨酸磷酸化，似乎在子宫收缩过程中发挥重要作用。这些研究试图确定哪些非受体蛋白酪氨酸激酶参与了大鼠子宫组织中磷脂酶C1的激活。利用同种型特异性蛋白酪氨酸激酶抑制剂进行体外收缩研究。利用磷酸酪氨酸磷脂酶C1、总磷脂酶C1、c-Src激酶和Lck激酶的抗体进行蛋白质印迹。自发的，拉伸刺激，和bpV（phen）（酪氨酸磷酸酶抑制剂）增强子宫收缩显着抑制响应Damnacanthal（Lck激酶抑制剂）和PP 1（c-Src激酶抑制剂）。虎刺醛和PP 1也显着抑制bpV（phen）增强磷脂酶C1的酪氨酸磷酸化。Western印迹证实Lck激酶和c-Src激酶在子宫组织中的表达。总之，Lck和c-Src激酶似乎在调节磷脂酶C1的酪氨酸磷酸化和大鼠子宫收缩活动中发挥重要作用。
Activated phospholipase C1, produced in response to tyrosine phosphorylation, appears to play an important role during uterine contractions. These studies sought to determine which non-receptor protein tyrosine kinases are involved in the activation of phospholipase C1 in rat uterine tissue. In vitro contraction studies were performed utilizing isoform specific protein tyrosine kinase inhibitors. Western blots were performed utilizing antibodies to phosphotyrosine-phospholipase C1, total phospholipase C1, c-Src kinase and Lck kinase. Spontaneous, stretch-stimulated, and bpV(phen) (tyrosine phosphatase inhibitor) enhanced uterine contractions were significantly suppressed in response to Damnacanthal (Lck kinase inhibitor) and PP1 (c-Src kinase inhibitor). Damnacanthal and PP1 also significantly suppressed bpV(phen)-enhanced tyrosine phosphorylation of phospholipase C1. Western blots confirmed expression of Lck kinase and c-Src kinase in uterine tissue. In conclusion, the Lck and c-Src kinases appear to play an important role in regulating tyrosine phosphorylation of phospholipase C1 and contractile activity in the rat uterus.
登录
查看更多内容
平滑肌拉伸激活磷脂酶 C 活性。
DOI：
10.1152/ajpcell.1995.268.2.c458
发表时间：
1995
期刊：
The American journal of physiology.
影响因子：
--
作者：
Matsumoto,H;Baron,CB;Coburn,RF
通讯作者：
Coburn,RF
磷脂酶 Cgamma1 酪氨酸磷酸化在阶段性子宫肌层收缩中的作用。
影响因子：
9.8
作者：
Phillippe,Mark;Sweet,LeighM;Engle,Daniel
通讯作者：
Engle,Daniel
妊娠和非妊娠大鼠子宫组织中磷脂酶C的蛋白表达。
DOI：
10.1067/mob.2001.118143
发表时间：
2001
期刊：
American journal of obstetrics and gynecology.
影响因子：
--
作者：
Kim,TT;Saunders,T;Bieber,E;Phillippe,M
通讯作者：
Phillippe,M
怀孕和非怀孕大鼠子宫肌组织中磷脂酰肌醇特异性磷脂酶 C 异构体的表达。
影响因子：
9.8
作者：
Bieber,E;Stratman,T;Sanseverino,M;Sangueza,J;Phillippe,M
通讯作者：
Phillippe,M