Nonequilibrium mechanics of active cytoskeletal networks
Nonequilibrium mechanics of active cytoskeletal networks
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DOI：
10.1126/science.1134404
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发表时间：
2007-01-19
期刊：
影响因子：
56.9
通讯作者：
MacKintosh, F. C.
中科院分区：
文献类型：
--
作者：
Mizuno, Daisuke;Tardin, Catherine;MacKintosh, F. C.
细胞通过其机械框架（细胞骨架）主动产生力并对力做出敏感反应，细胞骨架是一种包括聚合物和运动蛋白的非平衡复合材料。我们测量了由肌球蛋白 II、肌动蛋白丝和交联剂组成的简单三组分模型系统的动力学和机械特性。在该系统中，运动活动产生的应力控制了细胞骨架网络力学，将刚度增加了近 100 倍，并以三磷酸腺苷依赖性方式定性地改变了网络的粘弹性响应。我们提出了一个定量理论模型，将这种活性凝胶的大规模特性与分子力的产生联系起来。
Cells both actively generate and sensitively react to forces through their mechanical framework, the cytoskeleton, which is a nonequilibrium composite material including polymers and motor proteins. We measured the dynamics and mechanical properties of a simple three-component model system consisting of myosin II, actin filaments, and cross-linkers. In this system, stresses arising from motor activity controlled the cytoskeletal network mechanics, increasing stiffness by a factor of nearly 100 and qualitatively changing the viscoelastic response of the network in an adenosine triphosphate-dependent manner. We present a quantitative theoretical model connecting the large-scale properties of this active gel to molecular force generation.