Atomistic analysis of pseudoknotted RNA unfolding.
Atomistic analysis of pseudoknotted RNA unfolding.
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DOI：
10.1021/ja1109425
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发表时间：
2011-04
影响因子：
15
通讯作者：
Yujie Zhang;Jian Zhang;Wei Wang
中科院分区：
文献类型：
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作者：
Yujie Zhang;Jian Zhang;Wei Wang
作为重要的功能结构，RNA假结为研究二级和三级结构之间的相互作用以及三链体、非正则相互作用和同轴堆叠在折叠/展开过程中的作用提供了极好的模型。在这里，我们报告的第一个原子和统计分析的假结内的噬菌体T2的基因32 mRNA的展开。观察到多个解折叠途径，不同的过渡态，和各种中间结构。水分子被发现耦合到解折叠过程中通过驱逐或并发机制。
As important functional structures, RNA pseudoknots provide excellent models for studying the interplay between secondary and tertiary structures and the roles of triplexes, noncanonical interactions, and coaxial stacking in the folding/unfolding process. Here we report a first atomistic and statistical analysis of the unfolding of the pseudoknot within gene 32 mRNA of bacteriophage T2. Multiple unfolding pathways, diverse transition states, and various intermediate structures were observed. Water molecules were found to be coupled with the unfolding process via the expulsion or concurrent mechanism.