Characterization of Ventricular and Atrial Cardiomyocyte Subtypes from Human-Induced Pluripotent Stem Cells
Characterization of Ventricular and Atrial Cardiomyocyte Subtypes from Human-Induced Pluripotent Stem Cells
复制标题
人诱导多能干细胞心室和心房心肌细胞亚型的表征
DOI：
10.1007/978-1-0716-1484-6_14
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发表时间：
2021
影响因子：
--
通讯作者：
Yoshinori Yoshida
中科院分区：
文献类型：
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作者：
Misato Nakanishi-Koakutsu;Tadashi Takaki;Kenji Miki;Yoshinori Yoshida
人类iPSC衍生的心肌细胞（hiPSC-CM）有望用于心力衰竭的再生疗法和药物发现。hiPSC-CM主要是心室CM（VCM）以及心房CM（ACM）和起搏细胞的混合物。在这里，我们描述了一种方法，以丰富VCM和ACM分化，并通过基因表达分析，使用qRT-PCR和电生理特性，使用膜片钳方法来表征这些亚型。分化的VCM和ACM分别高表达VCM和ACM标记基因。此外，这两种亚型都显示出动作电位的特定特性。
Human iPSC-derived cardiomyocytes (hiPSC-CMs) are expected to be used in regenerative therapies and drug discovery for heart failure. hiPSC-CMs are a mixture of mainly ventricular CMs (VCMs) and also of atrial CMs (ACMs) and pacemaker cells. Here we describe a method to enrich VCM and ACM differentiation and to characterize these subtypes by gene expression analysis using qRT-PCR and by electrophysiological properties using the patch-clamp method. The differentiated VCMs and ACMs highly express VCM and ACM marker genes, respectively. Furthermore, both subtypes show specific properties of action potentials.