Two distinct domains in pro-region of Nodal-related 3 are essential for BMP inhibition
Two distinct domains in pro-region of Nodal-related 3 are essential for BMP inhibition
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DOI：
10.1016/j.bbrc.2006.05.121
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发表时间：
2006-07-28
影响因子：
3.1
通讯作者：
Asashima, Makoto
中科院分区：
文献类型：
--
作者：
Haramoto, Yoshikazu;Takahashi, Shuji;Asashima, Makoto
转化生长因子- β (tgf - β)超家族成员Xenopus nodal-related 3 （Xnr3）通过抑制骨形态发生蛋白（BMPs）诱导神经组织。我们最近发现了一种抑制机制，其中热带爪蟾节点相关3 （Xtnr3）的前区与BMP配体相互作用。在这里，我们发现二硫化物连接的异二聚化不会导致Xtnr3对BMP的抑制，并且Xtnr3成熟区域，其过表达可以诱导与全长Xtnr3相同的表型，不会抑制BMP信号传导。此外，我们发现Xtnr3的bmp抑制结构域分别位于亲区的N端和c端区域。这些结果表明，节点相关3的前区是其抑制BMP的必要和充分的。(c) 2006爱思唯尔公司版权所有。
The transforming growth factor-beta (TGF-beta) superfamily member, Xenopus nodal-related 3 (Xnr3), induces neural tissues through inhibition of bone morphogenetic proteins (BMPs). We recently identified an inhibitory mechanism in which the pro-region of Xenopus tropicalis nodal-related 3 (Xtnr3) physically interacts with BMP ligands. Here, we show that disulfide-linked heterodimerization does not contribute to BMP inhibition by Xtnr3 and that the Xtnr3 mature region, overexpression of which can induce the same phenotype as full-length Xtnr3, does not inhibit BMP signaling. Furthermore, we find that the BMP-inhibitory domains of Xtnr3 are separately located in the N- and C-terminal regions of the pro-region. These results indicate the pro-region of Nodal-related 3 is both necessary and sufficient for its BMP inhibition. (c) 2006 Elsevier Inc. All rights reserved.