Development of Genetic Diagnostic Methods for Detection for Novel Coronavirus 2019(nCoV-2019) in Japan
Development of Genetic Diagnostic Methods for Detection for Novel Coronavirus 2019(nCoV-2019) in Japan
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DOI：
10.7883/yoken.jjid.2020.061
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发表时间：
2020-07-01
影响因子：
2.2
通讯作者：
Takeda, Makoto
中科院分区：
文献类型：
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作者：
Shirato, Kazuya;Nao, Naganori;Takeda, Makoto
2019年底，中国武汉市出现新型冠状病毒2019（nCoV）爆发期间，许多航空旅客往返于武汉和日本之间，这表明日本人群感染该病毒的风险很高。因此，我们紧急开发了用于检测 2019 nCoV 的诊断系统。两种巢式 RT-PCR 和两种实时 RT-PCR 检测方法适用于日本。截至 2020 年 2 月 8 日，这些检测已在日本成功检测出 25 例阳性感染病例。
During the emergence of novel coronavirus 2019 (nCoV) outbreak in Wuhan city, China at the end of 2019, there was movement of many airline travelers between Wuhan and Japan, suggesting that the Japanese population was at high risk of infection by the virus. Hence, we urgently developed diagnostic systems for detection of 2019 nCoV. Two nested RT-PCR and two real-time RT-PCR assays were adapted for use in Japan. As of February 8, 2020, these assays have successfully detected 25 positive cases of infection in Japan.