Enhancement of neurite adhesion, alignment and elongation on conductive polypyrrole-poly(lactide acid) fibers with cell-derived extracellular matrix.
Enhancement of neurite adhesion, alignment and elongation on conductive polypyrrole-poly(lactide acid) fibers with cell-derived extracellular matrix.
复制标题
DOI：
10.1016/j.colsurfb.2016.10.014
复制
发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Xingxing Zhou;Anneng Yang;Zhongbing Huang;G. Yin;Ximing Pu;Juan Jin
中科院分区：
文献类型：
--
作者：
Xingxing Zhou;Anneng Yang;Zhongbing Huang;G. Yin;Ximing Pu;Juan Jin
细胞外基质(ECM)可促进周围神经修复。在本研究中，通过静电纺丝和电化学沉积聚吡咯纳米粒子，制备了具有芯-鞘结构的导电纤维膜，其导电性为∼10~S cm−1。然后通过培养和裂解L929细胞，将细胞外基质的多种成分，包括层粘连蛋白、纤维连接蛋白和胶原蛋白包覆在CFF表面，制成ECM-CFF生物活性支架(ECM-CFF)。100 mV/cm的电刺激(ES)14天，每天2小时，对ECM-CFF的电导率无明显影响。PC12细胞实验结果表明，细胞黏附率、轴突贴壁率和轴突定向率分别为∼95%、∼77%、∼70%，明显高于裸露CFF的17%、29%和14%。ECM-CFF组神经突起长度(∼79 mm)也大于裸露CFF组(∼25 mm)。ECM-CFF将100 mV/cm的ES注入PC12细胞，在培养的前3天能显著促进神经突起的生长。结果表明，ECM-CFF与ES联合应用可促进神经细胞间的黏附，促进轴突生长和延伸，从而促进神经再生。
Extracellular matrix (ECM) can promote peripheral nerve repair. In this study, a conductive fiber-film (CFF) with core-sheath structure and conductivity of ∼10 S cm−1was prepared by electrospinning of aligned poly(l-lactide acid) (PLLA) fibers and electrochemical deposition of polypyrole (PPy) nanoparticles. Then the multiple components of ECM, including laminin, fibronectin and collagen, were coated on the surface of CFF by culturing and lysing L929 cells to fabricate the bioactive scaffold of ECM-linked CFF (ECM-CFF). The electrical stimulation (ES) of 100 mV/cm for 14 days and 2 h per day did not significantly decrease the conductivity of ECM-CFF. The results of PC12 cells test indicated that, cells adhesion rate, neurite-bearing cell rate and neurite alignment rate on ECM-CFF were ∼95%, ∼77%, ∼70%, respectively, significantly larger than the corresponding values on bare CFF (17%, 29% and 14%, respectively). The neurites length on ECM-CFF (∼79 mm) was also larger than that on bare CFF (∼25 mm). ES of 100 mV/cm onto PC12 cells through ECM-CFF could significantly promote neurite extension in first 3 days of the neurite growth. These results indicated that, the combination of ECM-CFF with ES could improve the nerve regeneration by encouraging neural-cell adhesion, neurite growth and extension.