Different Astroglia Permissivity Controls the Migration of Olfactory Bulb Interneuron Precursors
Different Astroglia Permissivity Controls the Migration of Olfactory Bulb Interneuron Precursors
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DOI：
10.1002/glia.20918
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发表时间：
2010-01-15
期刊：
影响因子：
6.2
通讯作者：
Lopez-Mascaraque, Laura
中科院分区：
文献类型：
--
作者：
Garcia-Marques, Jorge;De Carlos, Juan A.;Lopez-Mascaraque, Laura
吻侧迁移流（RMS）是嗅球（OB）中间神经元前体细胞的一条明确的迁移途径。在RMS中，迁移细胞周围有密集的星形胶质细胞基质。然而，目前尚不清楚在何种程度上的星形胶质细胞基质参与迁移。在这里，我们分析了单层星形胶质细胞分离的区域，是宠物的移民（RMS和OB）和nonpermissive（皮质和邻近皮质区）培养成神经细胞的迁移行为。我们的研究结果表明，强大的成神经细胞迁移时，RMS外植体培养OB或RMS星形胶质细胞，在相反的行为对星形胶质细胞来自非许可地区。这些差异由星形胶质细胞衍生的不溶性因子介导，与细胞外基质和细胞粘附分子的过表达有关，如实时qRT-PCR所揭示的。我们的研究结果表明，星形胶质细胞的异质性可能发挥了重要作用，在RMS内的迁移和细胞分离的OB。(C)2009年威利-利斯。Inc
The rostral migratory stream (RMS) is a well defined migratory pathway for precursors of olfactory bulb (OB) interneurons Throughout the RMS an intense astroglial matrix surrounds the migratory cells. However, it is not clear to what extent the astroglial matrix participates in migration. Here, we have analyzed the migratory behavior of neuroblasts cultured on monolayers of astrocytes isolated from areas that are pet-missive (RMS and OB) and nonpermissive (cortex and adjacent cortical areas) to migration. Our results demonstrate robust neuroblast migration when RMS-explants are cultured on OB or RMS-astroclytes, in contrast to their behavior on astroglia derived from nonpermissive areas. These differences, mediated by astrocyte-derived nonsoluble factors, are related to the overexpression of extracellular matrix and cell adhesion molecules, as revealed by real-time qRT-PCR. Our results show that astroglia heterogeneity could play a significant role in migration within the RMS and in cell detachment in the OB. (C) 2009 Wiley-Liss. Inc