The Glycoprotein NOWA and Minicollagens Are Part of a Disulfidelinked Polymer That Forms the Cnidarian Nematocyst Wall*
The Glycoprotein NOWA and Minicollagens Are Part of a Disulfidelinked Polymer That Forms the Cnidarian Nematocyst Wall*
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糖蛋白NOWA 和微胶原是形成刺胞动物刺丝囊壁的二硫键连接聚合物的一部分*
DOI：
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发表时间：
2004
影响因子：
4.8
通讯作者：
T. Holstein
中科院分区：
文献类型：
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作者：
S. Özbek;E. Pokidysheva;M. Schwager;T. Schulthess;N. Tariq;Dirk Barth;A. Milbradt;L. Moroder;J. Engel;T. Holstein
刺胞囊是刺胞动物中参与防御和捕获猎物的独特的分泌细胞器。微胶原和糖蛋白NOWA是包囊囊壁的主要成分，其抵抗15 MPa的渗透压。在这里，我们提出了重组表达的NOWA，它自发地组装成球状大分子颗粒，是敏感的天然壁结构的减少。超微结构分析表明，水螅囊壁由若干层球状颗粒组成，这些颗粒通过放射状棒状突起相互连接。有证据表明，天然壁颗粒含有NOWA和微胶原，应该是通过其同源的富含半胱氨酸的结构域之间的二硫键连接。我们的数据表明，一个连续的超结构的包囊壁，组装从壁蛋白，共享一个共同的寡聚化基序。
The nematocyst is a unique extrusive organelle involved in the defense and capture of prey in cnidarians. Minicollagens and the glycoprotein NOWA are major components of the nematocyst capsule wall, which resists osmotic pressure of 15 MPa. Here we present the recombinant expression of NOWA, which spontaneously assembles to globular macromolecular particles that are sensitive to reduction as the native wall structure. Ultra-structural analysis showed that the Hydra nematocyst wall is composed of several layers of globular particles, which are interconnected via radiating rodlike protrusions. Evidence is presented that native wall particles contain NOWA and minicollagen, supposed to be linked via disulfide bonds between their homologous cysteine-rich domains. Our data suggest a continuous suprastructure of the nematocyst wall, assembled from wall proteins that share a common oligomerization motif.