Impaired virus-induced interferon-α2 release in adult asthmatic patients
Impaired virus-induced interferon-α2 release in adult asthmatic patients
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DOI：
10.1111/j.1365-2222.2006.02450.x
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发表时间：
2006-03-01
影响因子：
6.1
通讯作者：
Bufe, A
中科院分区：
文献类型：
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作者：
Gehlhar, K;Bilitewski, C;Bufe, A
背景干扰素-α不仅是免疫系统抵御病毒攻击的第一道防线，而且与辅助性T细胞1型(Th1)/辅助性T细胞2型(Th2)调节以及嗜碱性粒细胞和单核细胞等多种细胞类型相互作用，从而将先天免疫和获得性免疫联系起来。最近，我们发现过敏性哮喘儿童产生的病毒诱导的干扰素-α2的量明显低于健康儿童或内源性哮喘儿童。目的：在这项研究中，我们扩大了我们的分析范围，以检验成人过敏性哮喘患者的干扰素-α2是否也降低。结果过敏性哮喘患者外周血中病毒诱生的干扰素-α2显著低于健康对照组，且与病毒种类无关(呼吸道合胞病毒哮喘=221+/-134pg/mL，新城疫健康=555+/-341pg/mL，P=0.003；呼吸道合胞病毒哮喘=46+/-27pg/mL，新城疫健康=108+/-90pg/mL，P=0.014)。值=平均值+/-标准差)。它不受药物，特别是皮质类固醇的影响。新城疫病毒刺激5h后，干扰素-α2mRNA的表达证实了ELISA法的结果，并与释放数据有很好的相关性(r=0.397，P=0.033)。结论成人过敏性哮喘患者与儿童一样，存在病毒诱导的全血干扰素-α2m RNA释放降低，提示哮喘和特应性哮喘患者存在全身性现象。病毒诱导的干扰素-α释放受损可能是慢性过敏性哮喘炎症的一个标志。
Background Interferon-alpha (IFN-alpha) not only serves as a first defence line of the immune system against viral attacks but also interacts with T-helper type 1 (Th1)/ T-helper type 2 (Th2) regulation and various other cell types like basophils and monocytes, thereby linking innate and acquired immunity. Recently, we demonstrated that children with allergic asthma produced significantly lower amounts of virus-induced IFN-alpha 2 compared with healthy children or those with intrinsic asthma.Objective In this study, we extend our analysis to examine in a cohort study whether IFN-alpha 2 is also reduced in allergic asthma of adults.Methods Adults with allergic asthma and healthy controls were prospectively recruited. Blood cultures were stimulated with different viruses (respiratory syncytial virus (RSV), newcastle disease virus (NDV)) and analysed for IFN-alpha 2 protein release and gene transcription.Results Virus-induced IFN-alpha 2 release from blood cells of allergic asthmatic patients was significantly reduced compared with healthy controls, independent of the virus used (NDVasthma=221 +/- 134 pg/mL, NDVhealthy=555 +/- 341 pg/mL, P=0.003 and RSVasthma=46 +/- 27 pg/mL, RSVhealthy=108 +/- 90 pg/mL, P=0.014). Values=mean +/- standard deviation). It was not influenced by medication, especially cortico-steroids. IFN-alpha 2 mRNA expression 5 h after NDV stimulation confirmed the ELISA results and correlated well with release data (r=0.397, P=0.033).Conclusion Like children, adults with allergic asthma show impaired virus-induced IFN-alpha 2 release in whole blood, indicating a systemic phenomenon in patients with bronchial asthma and atopic phenotype. Impaired virus-induced IFN-alpha release could be a marker of inflammation in chronic allergic asthma.