Low concordance of oral and genital HPV infection among male patients with sexually transmitted infections in Vietnam
Low concordance of oral and genital HPV infection among male patients with sexually transmitted infections in Vietnam
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DOI：
10.1186/s12879-019-4175-0
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发表时间：
2019-07-04
影响因子：
3.7
通讯作者：
Ichimura, Hiroshi
中科院分区：
文献类型：
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作者：
Le, Hai Ha Long;Bi, Xiuqiong;Ichimura, Hiroshi
人类乳头瘤病毒（HPV）可导致男性癌症，包括阴茎癌、肛门癌和口咽癌.本横断面研究的目的是调查的患病率，基因型，HPV感染的危险因素在口腔中，相比，在生殖器中，男性诊断为性传播感染（STIs）在Vietnam.MethodsOral，尿液，阴茎，尿道样本收集198例男性越南性病患者（中位年龄31.0岁，范围17-68）。用改良的和/或原始的GP 5（+）/GP 6（+）引物进行PCR扩增。样本进行基因分型与基因阵列检测和/或人口sequencing.ResultsHPV DNA检测69（34.8%）的198例。其中，16名患者（8.1%）有口腔感染，58名患者（29.3%）有生殖器感染（4.5%在尿液中，25.8%在阴茎中，8.1%在尿道中）。口腔和生殖器之间HPV感染的一致性较差（kappa=0.01）。在16例口腔HPV DNA阳性的患者中，11例（68.8%）生殖器内无HPV DNA。在其余5名患者中，在两个部位都发现了HPV DNA，但只有1名患者在两个部位都显示出相似的毒株。在其他4例患者中，HPV基因型在这些部位之间完全不一致。HPV 18是口腔和生殖器部位最常见的高危型HPV，分别为9/16（56.3%）和10/58（17.2%）。多变量分析显示，（OR 1.05），高等教育（OR 2.17），不了解性传播感染（OR 4.21）是生殖道HPV感染的独立危险因素;相反，只有老年人结论口腔和生殖道感染部位HPV基因型一致性低，提示口腔HPV感染的获得性、持续性、和/或HPV感染的清除率在这些部位之间不同。虽然HPV DNA在口腔样本中的检出率明显低于生殖器样本，但口腔样本也应用于男性HPV筛查。
BackgroundHuman papillomavirus (HPV) causes cancers in men, including penile, anal, and oropharyngeal cancers. This cross-sectional study aimed to investigate the prevalence, the genotypes, and the risk factors of HPV infections in the oral cavity, compared to those in the genitals, among males diagnosed with sexually transmitted infections (STIs) in Vietnam.MethodsOral, urinary, penile, and urethral samples were collected from 198 male Vietnamese patients with STIs (median age 31.0years, range 17-68). HPV DNA was isolated and amplified with PCR, with modified and/or original GP5(+)/GP6(+) primers. Samples were genotyped with a gene array assay and/or population sequencing.ResultsHPV DNA was detected in 69 (34.8%) of 198 patients. Of these, 16 patients (8.1%) had infections in the oral cavity and 58 (29.3%) had infections in the genitals (4.5% in the urine, 25.8% in the penis, and 8.1% in the urethra). The concordance of HPV infections between the oral cavity and the genitals was poor (kappa=0.01). Of the 16 patients with oral HPV DNA, 11 (68.8%) had no HPV DNA in the genitals. In the remaining five patients, HPV DNA was found at both sites, but only one showed similar strains at both sites. In the other four patients, the HPV genotypes were completely discordant between these sites. HPV18 was the most common high-risk HPV genotype in both oral (9/16, 56.3%) and genital (10/58, 17.2%) sites. Multivariable analyses showed that older age (OR 1.05), higher education (OR 2.17), and no knowledge of STIs (OR 4.21) were independent risk factors for genital HPV infections; in contrast, only older age (OR 1.05) was an independent risk factor for oral HPV infections.ConclusionsThe low concordance of HPV genotypes between oral and genital infection sites suggested that the acquisition, persistence, and/or clearance of HPV infections were different between these sites. Although HPV DNA was detected significantly less frequently in oral samples than in genital samples, oral samples should also be used for HPV screening in men.