Characterization of class II alpha genes and DLA-D region allelic associations in the dog.
Characterization of class II alpha genes and DLA-D region allelic associations in the dog.
复制标题
狗的 II 类 α 基因和 DLA-D 区域等位基因关联的表征。
DOI：
10.1111/j.1399-0039.1988.tb01660.x
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Storb,RF
中科院分区：
文献类型：
--
作者：
Sarmiento,UM;Storb,RF
用人类主要组织相容性复合体(HLA)基因探针分析犬DLA-D区α基因的限制性内切酶长度多态(RFLP)。用限制性内切酶(BamHI、EcoRI、Hind III、Pvu II、Taq I、Rsa I、Msp I、Pst I和Bgl II)对23只DJA-D纯合犬外周血白细胞DNA进行限制性内切酶消化，经琼脂糖凝胶电泳法分离后转移到生物示踪膜上。将Southern杂交结果与相应于DQ、DP、DZ和DRα基因的放射性标记的HLAc DNA探针杂交。明确的证据表明，狗的DO和DRα基因的同源物分别具有简单的双等位或三等位基因多态。还获得了单一、非多态的DPα基因的证据。然而，在犬基因组DNA中不能清楚地证明DZα基因的存在。这份报告扩展了我们先前的FWLP分析，记录了DLA II类β基因在同一组同型细胞犬中的多态性，并为在群体水平上进行DLA-D基因分型提供了基础。这项研究还表征了九种不同纯合分型细胞特异性中跨DLA-D区域的RFLP定义的优先等位基因关联。
Human major histocompatibility complex (HLA) cDNA probes were used to analyze the restriction hagment length polymorphism (RFLP) of the alpha genes of the DLA‐D region in dogs. Genomic DNA horn peripheral blood leucocytes of 23 unrelated DJA‐D homozygous dogs representing nine DLA‐D types (defined by mixed leucocyte reaction) was digested with restriction enzymes (BamHI, EcoRI, Hind III, Pvu II, Taq I, Rsa I, Msp I, Pst I and Bgl II), separated by agarose gel electrophoresis and transferred onto Biotrace membrane. The Southern blots were successively hybridized with radiolabelled HLA cDNA probes corresponding to DQ, DP, DZ and DR alpha genes. Clear evidence was obtained for the canine homologues of DO and DR alpha genes with simple bi‐ or tri‐allelic polymorphism, respectively. Evidence for a single, nonpolymorphic DP alpha gene was also obtained. However, the presence of a DZ alpha gene could not be clearly demonstrated in canine genomic DNA. This report extends our previous FWLP analysis documenting polymorphism of DLA class II beta genes in the same panel of homoygous typing cell dogs, and provides the basis for DLA‐D genotyping at a population level. This study also characterizes the RFLP‐defined preferential allelic associations across the DLA‐D region in nine different homozygous typing cell specificities.