Induced phenylamide accumulation in response to pathogen infection and hormone treatment in rice (Oryza sativa)
Induced phenylamide accumulation in response to pathogen infection and hormone treatment in rice (Oryza sativa)
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DOI：
10.1080/09168451.2018.1429889
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发表时间：
2018-01-01
影响因子：
1.6
通讯作者：
Ishihara, Atsushi
中科院分区：
文献类型：
--
作者：
Morimoto, Noriko;Ueno, Kotomi;Ishihara, Atsushi
水稻植物会积累各种特殊的代谢物，包括苯酰胺，以应对病原体的攻击。我们制备了 25 种苯基酰胺，并开发了一种通过液相色谱与串联质谱联用的多重反应监测来分析它们的方法。我们分析了感染 Cochliobolus miyabeanus 和 Xanthomonas oryzae 的水稻叶片中的苯酰胺。诱导的苯酰胺包括苯甲酰色胺、肉桂酰-、对香豆酰-、阿魏酰-和苯甲酰血清素、肉桂酰和苯甲酰酪胺、阿魏酰基丁胺和阿魏酰腐胺。一些苯酰胺对 C. miyabeanus 和 X. oryzae 表现出抗菌活性，表明它们是植物抗毒素。用茉莉酸、水杨酸、6-苄氨基嘌呤和乙烯利处理也诱导苯酰胺积累。诱导的酰胺的组成根据所使用的植物激素而变化，肉桂酰色胺、肉桂酰血清素和肉桂酰酪胺不是由植物激素诱导的。这些发现表明，几种植物激素和其他因素参与了水稻中响应病原体感染的苯酰胺积累。
Rice plants accumulate various specialized metabolites, including phenylamides, in response to pathogen attack. We prepared 25 phenylamides, and developed a method of analyzing them by multiple reaction monitoring with liquid chromatography coupled with tandem mass spectrometry. We analyzed phenylamides in rice leaves infected with Cochliobolus miyabeanus and Xanthomonas oryzae. The phenylamides induced included benzoyltryptamine, cinnamoyl-, p-coumaroyl-, feruloyl-, and benzoylserotonins, cinnamoyl and benzoyltyramines, feruloylagmatine, and feruloylputrescine. Some of the phenylamides exhibited antimicrobial activity against C. miyabeanus and X. oryzae, indicating that they are phytoalexins. Treatment with jasmonic acid, salicylic acid, 6-benzylaminopurine, and ethephone also induced phenylamide accumulation. The compositions of the induced amides varied depending on the plant hormone used, and cinnamoyltryptamine, cinnamoylserotonin, and cinnamoyltyramine were not induced by the plant hormones. These findings suggest that several plant hormones and additional factors are involved in phenylamide accumulation in response to pathogen infection in rice.