Expression of p21WAF1/CIP1 during mouse odontogenesis
Expression of p21WAF1/CIP1 during mouse odontogenesis
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DOI：
10.1111/j.1600-0722.1998.tb02160.x
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发表时间：
1998-01-01
影响因子：
1.9
通讯作者：
Ruch, JV
中科院分区：
文献类型：
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作者：
Bloch-Zupan, A;Leveillard, T;Ruch, JV
p21（WAF1/CIP1）是细胞周期蛋白依赖性激酶（Cdk）抑制剂。该蛋白可能在发育过程中作为一种诱导生长抑制剂起作用，有助于细胞周期退出和分化。p21在小鼠胚胎发生过程中的表达模式与骨骼肌、软骨、皮肤和鼻上皮等多种细胞系的终末分化有关。通过原位杂交分析P21在含维甲酸或不含维甲酸的化学定义培养基中在牙形成和离体牙齿发育过程中的表达。P21转录本在牙帽期晚期和钟形期初期局限于内牙上皮区域，然后局限于有丝分裂后的成牙细胞和成釉细胞。正在复制的细胞没有任何信号。无论培养条件如何，p21 mRNA在体外的分布与体内相似。P21蛋白的免疫定位叠加在转录本分布上，但在成釉细胞中更受限制。已知TGF β 1可诱导p21表达。在牙细胞分化过程中，TGF β 1和p21表达重叠。TGF β 1抑制生长可能与p21诱导有关。
p21(WAF1/CIP1) is a cyclin-dependent kinase (Cdk) inhibitor. This protein may function during development as an inducible growth inhibitor that contributes to cell cycle exit and differentiation. The expression pattern of p21 during mouse embryogenesis was correlated with terminal differentiation of multiple cell lineages including skeletal muscles, cartilage, skin and nasal epithelium. p21 expression was analyzed by in situ hybridization during odontogenesis as well as during in vitro tooth development in chemically defined medium with or without retinoic acid. p21 transcripts were detected in the restricted area of inner dental epithelium during late cap and initial bell stages and then confined to the post-mitotic odontoblasts and ameloblasts. The replicating cells were devoid of any signal. The distribution of p21 mRNA in vitro, whatever the culture conditions, was similar to the in vivo pattern. p21 protein immunolocalization was superimposed on the transcripts distribution but more restricted in ameloblasts. TGF beta 1 is known to induce p21 expression. During dental cytodifferentiations, TGF beta 1 and p21 expressions overlap. Growth inhibition by TGF beta 1 may be associated with p21 induction.