GH3-mediated auxin homeostasis links growth regulation with stress adaptation response in Arabidopsis
GH3-mediated auxin homeostasis links growth regulation with stress adaptation response in Arabidopsis
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DOI：
10.1074/jbc.m610524200
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发表时间：
2007-03-30
影响因子：
4.8
通讯作者：
Park, Chung-Mo
中科院分区：
文献类型：
--
作者：
Park, Jung-Eun;Park, Ju-Young;Park, Chung-Mo
植物不断监测环境波动以优化其生长和新陈代谢。一个例子是适应性生长发生在生物和非生物应力。在这里，我们表明，生长激素3介导的生长素稳态是一个重要组成部分的复杂网络的生长素行动，调节胁迫适应反应在拟南芥。内源生长素库至少部分地通过负反馈由一组生长素诱导的编码生长素缀合酶的GH 3基因调节。拟南芥突变体wes 1-D，其中GH 3基因WES 1被附近插入的S-35增强子激活，表现出生长素缺乏的性状，包括生长减少和叶形改变。有趣的是，WES 1也被各种胁迫条件以及水杨酸和脱落酸诱导。相应地，wes 1-D对生物和非生物胁迫都具有抗性，并且胁迫响应基因如病程相关基因和CBF基因在该突变体中上调。相反，T-DNA插入突变体表现出降低的抗逆性。因此，我们建议，生长激素3介导的生长抑制指导重新分配的代谢资源，以建立抗性和代表的健身成本诱导的阻力。
Plants constantly monitor environmental fluctuations to optimize their growth and metabolism. One example is adaptive growth occurring in response to biotic and abiotic stresses. Here, we demonstrate that GH3-mediated auxin homeostasis is an essential constituent of the complex network of auxin actions that regulates stress adaptation responses in Arabidopsis. Endogenous auxin pool is regulated, at least in part, through negative feedback by a group of auxin-inducible GH3 genes encoding auxin-conjugating enzymes. An Arabidopsis mutant, wes1-D, in which a GH3 gene WES1 is activated by nearby insertion of the S-35 enhancer, exhibited auxin-deficient traits, including reduced growth and altered leaf shape. Interestingly, WES1 is also induced by various stress conditions as well as by salicylic acid and abscisic acid. Accordingly, wes1-D was resistant to both biotic and abiotic stresses, and stress-responsive genes, such as pathogenesis-related genes and CBF genes, were upregulated in this mutant. In contrast, a T-DNA insertional mutant showed reduced stress resistance. We therefore propose that GH3-mediated growth suppression directs reallocation of metabolic resources to resistance establishment and represents the fitness costs of induced resistance.