Co-expression of the 5-HT3B subunit with the 5-HT3A receptor reduces alcohol sensitivity
Co-expression of the 5-HT3B subunit with the 5-HT3A receptor reduces alcohol sensitivity
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DOI：
10.1016/j.molbraines.2005.09.011
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发表时间：
2005-12-14
期刊：
影响因子：
--
通讯作者：
Machu, TK
中科院分区：
文献类型：
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作者：
Hayrapetyan, V;Jenschke, M;Machu, TK
采用全细胞膜片钳电生理记录在HEK 293细胞中评估了乙醇和三氯乙醇（TCEt）对小鼠5-羟色胺（3A）（5-HT 3A）和5-HT 3A/B受体功能的变构调节。乙醇增强5-HT 3A受体功能，但对小鼠5-HT 3A/B受体介导的电流无影响。三氯乙醇（TCEt）对小鼠5-HT 3A/B受体功能的增强作用远小于对小鼠5-HT 3A受体的增强作用。在观察到酒精诱导的峰值振幅增加的情况下，电流起始的20-80%上升相的斜率也增强，这表明激活速率的增加可能是酒精增强5-HT 3受体功能的一种机制。(c)2005 Elsevier B. V.保留所有权利。
Allosteric modulation of mouse 5-Hydroxytryptamine(3A) (5-HT3A) and 5-HT3A/B receptor function by ethanol and trichloroethanol (TCEt) was assessed in HEK293 cells with whole cell patch-clamp electrophysiological recordings. Ethanol enhanced 5-HT3A receptor function, but had no effect on mouse 5-HT3A/B receptor mediated currents. The enhancing action of trichloroethanol (TCEt) on mouse 5-HT3A/B receptor function was much less than that observed in the mouse 5-HT3A receptor. Where alcohol-induced increases in peak amplitude were observed, the slope of the 20-80% rising phase of current onset was also enhanced, suggesting that increases in activation rate may be one mechanism through which alcohols enhance function of the 5-HT3 receptors. (c) 2005 Elsevier B.V. All rights reserved.