The taxonomic composition of the donor intestinal microbiota is a major factor influencing the efficacy of faecal microbiota transplantation in therapy refractory ulcerative colitis.
The taxonomic composition of the donor intestinal microbiota is a major factor influencing the efficacy of faecal microbiota transplantation in therapy refractory ulcerative colitis.
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DOI：
10.1111/apt.14387
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发表时间：
2018-01
影响因子：
7.6
通讯作者：
Högenauer C
中科院分区：
文献类型：
--
作者：
Kump P;Wurm P;Gröchenig HP;Wenzl H;Petritsch W;Halwachs B;Wagner M;Stadlbauer V;Eherer A;Hoffmann KM;Deutschmann A;Reicht G;Reiter L;Slawitsch P;Gorkiewicz G;Högenauer C
粪便微生物群移植是治疗溃疡性结肠炎患者的实验方法。虽然越来越多的证据表明粪便微生物群移植对这种疾病有效，但影响其反应的因素尚不清楚。在溃疡性结肠炎患者中建立粪便微生物群移植治疗方案，并研究哪些患者或供体因素是治疗成功的原因。这是一项在27名治疗难治性溃疡性结肠炎患者中进行的90天以上抗生素预治疗（FMT组，n = 17）与仅抗生素预治疗（AB组，n = 10）后重复粪便微生物群移植的开放对照试验。通过基于16 SrRNA基因的微生物群分析来分析供体和患者的粪便样本。在FMT组中，10/17（59%）的患者对粪便微生物群移植有反应，4/17（24%）的患者缓解。对粪便微生物群移植的反应主要受供体微生物群的分类组成的影响。具有高细菌丰度（在15367 rps下观察到的物种缓解946 ± 93 vs无响应797 ± 181）和嗜粘蛋白阿克曼氏菌（3.3 ± 3.1% vs 0.1 ± 0.2%）、未分类瘤胃球菌科（13.8 ± 5.0% vs 7.5 ± 3.7%）和瘤胃球菌属（Ruminococcus spp.）的高相对丰度的供体粪便。(4.9± 3.5% vs 1.0 ± 0.7%）更可能诱导缓解。相比之下，单独抗生素治疗（AB组）耐受性较差，可能是因为肠道微生物丰富度持续下降。供体肠道微生物群的分类组成是影响溃疡性结肠炎患者粪便微生物群移植疗效的主要因素。特定微生物制剂的设计可能会导致溃疡性结肠炎的新治疗方法。
Faecal microbiota transplantation is an experimental approach for the treatment of patients with ulcerative colitis. Although there is growing evidence that faecal microbiota transplantation is effective in this disease, factors affecting its response are unknown. To establish a faecal microbiota transplantation treatment protocol in ulcerative colitis patients, and to investigate which patient or donor factors are responsible for the treatment success. This is an open controlled trial of repeated faecal microbiota transplantation after antibiotic pre‐treatment (FMT‐group, n = 17) vs antibiotic pre‐treatment only (AB‐group, n = 10) in 27 therapy refractory ulcerative colitis patients over 90 days. Faecal samples of donors and patients were analysed by 16SrRNA gene‐based microbiota analysis. In the FMT‐group, 10/17 (59%) of patients showed a response and 4/17 (24%) a remission to faecal microbiota transplantation. Response to faecal microbiota transplantation was mainly influenced by the taxonomic composition of the donor's microbiota. Stool of donors with a high bacterial richness (observed species remission 946 ± 93 vs no response 797 ± 181 at 15367 rps) and a high relative abundance of Akkermansia muciniphila (3.3 ± 3.1% vs 0.1 ± 0.2%), unclassified Ruminococcaceae (13.8 ± 5.0% vs 7.5 ± 3.7%), and Ruminococcus spp. (4.9 ± 3.5% vs 1.0 ± 0.7%) were more likely to induce remission. In contrast antibiotic treatment alone (AB‐group) was poorly tolerated, probably because of a sustained decrease of intestinal microbial richness. The taxonomic composition of the donor's intestinal microbiota is a major factor influencing the efficacy of faecal microbiota transplantation in ulcerative colitis patients. The design of specific microbial preparation might lead to new treatments for ulcerative colitis.
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影响因子：
7.4
作者：
Cui B;Li P;Xu L;Zhao Y;Wang H;Peng Z;Xu H;Xiang J;He Z;Zhang T;Nie Y;Wu K;Fan D;Ji G;Zhang F
通讯作者：
Zhang F
DOI：
10.1038/ismej.2012.8
发表时间：
2012-08
期刊：
The ISME journal
影响因子：
--
作者：
通讯作者：
--
影响因子：
29.4
作者：
Gionchetti, P;Rizzello, F;Campieri, M
通讯作者：
Campieri, M
影响因子：
4.9
作者：
Kump, Patrizia K.;Groechenig, Hans-Peter;Hoegenauer, Christoph
通讯作者：
Hoegenauer, Christoph
影响因子：
39.2
作者：
Kelly CR;Khoruts A;Staley C;Sadowsky MJ;Abd M;Alani M;Bakow B;Curran P;McKenney J;Tisch A;Reinert SE;Machan JT;Brandt LJ
通讯作者：
Brandt LJ