Identification of temporally and spatially phosphate-starvation responsive genes in Glycine max
Identification of temporally and spatially phosphate-starvation responsive genes in Glycine max
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DOI：
10.1016/j.plantsci.2008.06.007
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发表时间：
2008-10-01
期刊：
影响因子：
5.2
通讯作者：
Yan, Xiaolong
中科院分区：
文献类型：
--
作者：
Guo, Wenbing;Zhang, Lina;Yan, Xiaolong
低磷是制约大豆生产的主要因素之一。Merr.），是世界上重要的豆科作物。为了更好地了解大豆适应低磷胁迫的分子机制，本研究以磷高效大豆基因型BX 10为材料，通过抑制性消减杂交技术从4个cDNA文库中筛选磷饥饿反应基因。结果表明，在短期（0.5-12 h）和长期（3-12 d）磷饥饿（0.2 μ M Pi）处理的大豆根和地上部中，共筛选出215个差异表达基因。这些基因被分为五组，分别代表代谢、遗传信息处理、与环境的相互作用、发育和未知功能。比较四个文库中属于不同类别的基因的相对比例，表明这些基因具有不同的时空表达模式。根中的许多基因的转录被短期磷饥饿诱导，但在地上部中没有。此外，根长期文库中最大的基因组属于遗传信息处理类别，而茎长期文库中最大的基因组涉及代谢，表明大豆的茎和根对长期磷饥饿的反应不同。通过实时定量PCR分析进一步证实了属于不同文库的12个基因的差异表达。我们的研究表明，不同的基因在空间和时间整合在大豆植物响应磷饥饿。(c)2008爱思唯尔爱尔兰有限公司保留所有权利。
Low phosphorus (P) availability is a major constraint to the production of soybean (Glycine max (L.) Merr.), an important leguminous crop in the world. To gain a better insight into the molecular mechanisms by which soybean adapts to low P availability, a P-efficient soybean genotype, BX10, was used to identify phosphate-starvation responsive genes from four cDNA libraries constructed by suppression subtractive hybridization. The results showed that 215 represented genes selected were differentially expressed in the roots and shoots of soybean subjected to either short-term (0.5-12 h) or long-term (3-12 days) Pi starvation (0.2 mu M Pi). The selected genes were categorized into five groups representing Metabolism, Genetic Information Processing, Interaction with the Environment, Development, and Unknown Function. Comparison of the relative proportion of genes belonging to different categories in the four libraries indicated distinct spatial and temporal expression patterns of the genes. Transcripts of many genes were induced by short-term Pi starvation in roots, but not in shoots. Furthermore, the largest group of genes from the root long-term library was under the Genetic Information Processing category, whereas the largest group from shoot long-term library was involved in Metabolism, indicating that shoots and roots of soybeans respond differently to long-term Pi starvation. Differential expression of twelve genes belonging to different libraries was further confirmed by quantitative real-time PCR analysis. Our study has demonstrated that various genes are spatially and temporally integrated in soybean plant in response to Pi starvation. (c) 2008 Elsevier Ireland Ltd. All rights reserved.