A new C-type cyclin-dependent kinase from tomato expressed in dividing tissues does not interact with mitotic and G1 cyclins
A new C-type cyclin-dependent kinase from tomato expressed in dividing tissues does not interact with mitotic and G1 cyclins
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DOI：
10.1104/pp.126.4.1403
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发表时间：
2001-08-01
期刊：
影响因子：
7.4
通讯作者：
Chevalier, C
中科院分区：
文献类型：
--
作者：
Joubés, J;Lemaire-Chamley, M;Chevalier, C
细胞周期蛋白依赖性激酶（CDK）形成真核生物丝氨酸-苏氨酸蛋白激酶的保守超家族，其活性需要细胞周期蛋白的结合。CDK参与细胞生物学的许多方面，特别是细胞周期的调控。从番茄（Lycopsersicon esculentum Mill.）并命名为Lyces; CDKC;1（登录号AJ 294903.），Lyces; CDKB 1;1（登录号AJ 297916）和Lyces; CDKB 2;1（登录号AJ 297917）。预测的氨基酸序列在细胞周期蛋白结合结构域中显示特征性PITAIRE（CDKC）、PPTALRE（CDKB 1）和PPTTLRE（CDKB 2）基序，清楚地识别了CDK的类型。所有的成绩单的积累优先与发展中的番茄果实和营养器官的分裂组织。与CDKA和CDKBs相反，在番茄细胞悬浮培养和离体根中，Lyces;CDKC;1的转录模式被证明是独立的激素和糖供应。这一观察结果，连同在原位杂交实验中不存在斑片状表达谱，表明Lyces;CDKC;I的非细胞周期调节。使用双杂交试验，我们表明，Lyces;CDKC;1不与有丝分裂和G1期细胞周期蛋白相互作用。植物CDKCs在细胞分裂和分化的调节中的作用进行了讨论，关于其动物对应物的已知功能。
Cyclin-dependent kinases (CDKs) form a conserved superfamily of eukaryotic serine-threonine protein kinases whose activity requires the binding of a cyclin protein. CDKs are involved in many aspects of cell biology and notably in the regulation of the cell cycle. Three DNAs encoding a C-type CDK, and a member of each B-type CDK subfamily, were isolated from tomato (Lycopsersicon esculentum Mill.) and designated Lyces; CDKC;1 (accession no. AJ294903.), Lyces; CDKB1;1 (accession no. AJ297916), and Lyces;CDKB2;1 (accession no, AJ297917). The predicted amino acid sequences displayed the characteristic PITAIRE (CDKC), PPTALRE (CDKB1), and PPTTLRE (CDKB2) motives in the cyclin-binding domain, clearly identifying the type of CDK. The accumulation of all transcripts was associated preferentially with dividing tissues in developing tomato fruit and vegetative organs. In contrast to that of CDKA and CDKBs, the transcription pattern of Lyces;CDKC;1 was shown to be independent of hormone and sugar supply in tomato cell suspension cultures and excised roots. This observation, together with the absence of a patchy expression profile in in situ hybridization experiments, suggests a non-cell cycle regulation of Lyces;CDKC;I. Using a two-hybrid assay, we showed that Lyces;CDKC;1 did not interact with mitotic and G1 cyclins. The role of plant CDKCs in the regulation of cell division and differentiation is discussed with regard to the known function of their animal counterparts.