Hepatitis C virus RNA in dried serum spotted onto filter paper is stable at room temperature
Hepatitis C virus RNA in dried serum spotted onto filter paper is stable at room temperature
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DOI：
10.1128/jcm.36.10.3070-3072.1998
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发表时间：
1998-10-01
影响因子：
9.4
通讯作者：
Konomi, N
中科院分区：
文献类型：
--
作者：
Abe, K;Konomi, N
为了克服病毒RNA的不稳定性，我们将丙型肝炎病毒（HCV） RNA检测在干燥的血清中，在滤纸上斑点，斑点血清样品tr；结果表明，HCV RNA在干燥条件下是稳定的，因为在室温下保存1周的斑点血清样品中可以检测到。此外，尽管在室温下保存4周的干燥血清中HCV RNA滴度在病毒场中下降了10倍，但冷冻血清样品和干燥血清样品的PCR结果完全匹配，这种储存方法即使在没有冷冻条件的情况下也便于核酸扩增技术的运输和分析。
To overcome the instability of viral RNA, we carried out hepatitis C virus (HCV) RNA detection in dried serum spotted onto filter paper, The spotted serum samples tr;ere stored at room temperature and then processed for FCR assay at intervals of 1, 2, 3, and 4 weeks, Thf results shelved that serum HCV RNA is stable in a dried condition, as it was detectable in spotted serum samples stored far 1 weeks at room temperature. Furthermore, although the HCV RNA titer showed an similar to 10-fold reduction in virus field in dried serum stored at room temperature for 4 weeks, the PCR results of frozen serum samples and dried serum samples matched completely, This storage method facilitates transport and analysis by nucleic acid amplification techniques even when freezing conditions are not available.