EFFECTS OF PROTEIN-SYNTHESIS INHIBITION ON MEMORY FOR ACTIVE AVOIDANCE TRAINING
EFFECTS OF PROTEIN-SYNTHESIS INHIBITION ON MEMORY FOR ACTIVE AVOIDANCE TRAINING
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DOI：
10.1016/0031-9384(75)90163-8
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发表时间：
1975-01-01
影响因子：
2.9
通讯作者：
ROSENZWEIG, MR
中科院分区：
文献类型：
--
作者：
FLOOD, JF;BENNETT, EL;ROSENZWEIG, MR
在小鼠中研究了茴香霉素和乙酰氧基放线菌酮对脑蛋白合成的抑制作用，以了解其生化和行为效应。通过在一系列注射中以低剂量使用这两种药物，我们能够在长达 14 小时内维持 80% 或更高的蛋白质合成抑制，而不会造成可检测到的永久性生理损伤。这些药物被用作在 T 迷宫中训练以避免足部电击的小鼠的记忆删除剂。随着抑制持续时间的增加，失忆小鼠的比例增加。这种失忆症可以通过增加（a）习得率或（b）训练试验的次数来减少。茴香霉素被证明会导致对学习中的逃避成分和回避成分的显着程度的遗忘。与注射生理盐水的对照相比，训练前 15 分钟单次注射茴香霉素不会导致回避条件反射的获得或保留发生显着变化。只有在训练后额外注射以延长抑制时间才会导致失忆。因此，那些对获得遗忘症至关重要的注射是在不可能发生干扰记忆的时间进行的，因此结果与记忆过程有明确的关系。
Inhibition of brain protein synthesis by anisomycin and acetoxycycloheximide was studied in mice for its biochemical and behavioral effects. By employing both drugs in low doses in a series of injections, we were able to maintain inhibition of protein synthesis of 80% or greater for up to 14 hr without causing detectable pemanent physiological impaiment. The drugs were employed as amnestic agents in mice trained to avoid footshock in a T-maze. As the duration of inhibition increased, the percentage of mice classed as amnesic increased. This amnesia could be reduced by increasing (a) the rate of acquisition, or (b) the number of training trials. Anisomycin was shown to cause a significant degree of amnesia for the escape component as well as the avoidance component of the learning. A single injection of anisomycin given 15 min prior to training did not cause significant changes in the acquisition or retention of avoidance conditioning, when comparison was made with saline-injected controls. Only additional injections given after training to prolong inhibition caused amnesia. Thus, those injections critical in obtaining amnesia were given at a time at which interference with acquisition could not have occurred, so the results bear clearly on memory processes.