Isolation, Characterization and Functional Examination of the Gingival Immune Cell Network.
Isolation, Characterization and Functional Examination of the Gingival Immune Cell Network.
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DOI：
10.3791/53736
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发表时间：
2016-02-16
期刊：
影响因子：
--
通讯作者：
Moutsopoulos NM
中科院分区：
文献类型：
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作者：
Dutzan N;Abusleme L;Konkel JE;Moutsopoulos NM
组织中的免疫细胞网络在介导局部免疫和维持组织稳态中起着至关重要的作用，但对口腔黏膜和牙龈中的常驻免疫细胞群知之甚少。我们已经建立了一种从小鼠牙龈组织中分离和研究免疫细胞的技术，牙龈组织是微生物持续暴露的区域，也是常见炎症性疾病牙周炎的易感部位。我们的方案允许一个详细的表型表征免疫细胞群驻留在牙龈，即使在稳定的状态。我们的方法也产生了足够的高活力细胞，用于功能研究，如体外细胞因子分泌的评估。牙龈免疫细胞网络的表型和功能特征的结合将有助于研究口腔免疫和牙周稳态的机制，但也将促进我们对局部免疫病理机制的理解。
Immune cell networks in tissues play a vital role in mediating local immunity and maintaining tissue homeostasis, yet little is known of the resident immune cell populations in the oral mucosa and gingiva. We have established a technique for the isolation and study of immune cells from murine gingival tissues, an area of constant microbial exposure and a vulnerable site to a common inflammatory disease, periodontitis. Our protocol allows for a detailed phenotypic characterization of the immune cell populations resident in the gingiva, even at steady state. Our procedure also yields sufficient cells with high viability for use in functional studies, such as the assessment of cytokine secretion ex vivo. This combination of phenotypic and functional characterization of the gingival immune cell network should aid towards investigating the mechanisms involved in oral immunity and periodontal homeostasis, but will also advance our understanding of the mechanisms involved in local immunopathology.