MULTIPLICITY OF AMINO ACID PERMEASES IN SACCHAROMYCES-CEREVISIAE .4. EVIDENCE FOR A GENERAL AMINO ACID PERMEASE
MULTIPLICITY OF AMINO ACID PERMEASES IN SACCHAROMYCES-CEREVISIAE .4. EVIDENCE FOR A GENERAL AMINO ACID PERMEASE
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DOI：
10.1128/jb.103.3.770-777.1970
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发表时间：
1970-01-01
影响因子：
3.2
通讯作者：
CRABEEL, M
中科院分区：
文献类型：
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作者：
GRENSON, M;HOU, C;CRABEEL, M
动力学和遗传学证据表明，除了特定的氨基酸通透酶，酵母菌有一个通用的氨基酸通透酶，催化碱性和中性氨基酸的运输，但最有可能不是脯氨酸。一般的氨基酸通透酶似乎是组成型的，当铵离子被添加到培养基中时，其活性被抑制。分离到一个丧失了一般氨基酸通透酶活性的突变体。它的突变，命名为gap（通用氨基酸通透酶），与Surdin等人的aap（氨基酸通透酶）突变不等位，其具有完全不同的表型，不能认为其选择性地丧失了一般的氨基酸通透酶活性。
Kinetic and genetic evidences are presented to show that, in addition to specific amino acid permeases,Saccharomyces cerevisiaehas a general amino acid permease which catalyzes the transport of basic and neutral amino acids, but most probably not that of proline. The general amino acid permease appears to be constitutive, and its activity is inhibited when ammonium ions are added to the culture medium. A mutant which has lost the general amino acid permease activity was isolated. Its mutation, namedgap(general amino acid permease), is not allelic to theaap(amino acid permease) mutation of Surdin et al., which has a quite different phenotype and cannot be considered as having selectively lost the general amino acid permease activity.