Monocyte heterogeneity underlying phenotypic changes in monocytes according to SIV disease stage
Monocyte heterogeneity underlying phenotypic changes in monocytes according to SIV disease stage
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DOI：
10.1189/jlb.0209082
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发表时间：
2010-04-01
影响因子：
5.5
通讯作者：
Williams, Kenneth
中科院分区：
文献类型：
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作者：
Kim, Woong-Ki;Sun, Yue;Williams, Kenneth
HIV感染与表达CD 16抗原的单核细胞的扩增有关，但这在HIV发病机制中的意义在很大程度上是未知的。在恒河猴中，基于其CD 14和CD 16的表达，至少鉴定了三种血液单核细胞亚群：CD 14（高）CD 16（-）、CD 14（高）CD 16（低）和CD 14（低）CD 16（高）。这些亚群的表型和功能，包括CD 16（+）单核细胞，在正常的，未感染的恒河猴和猕猴感染SIV或嵌合SHIV。为了评估这些不同的单核细胞亚群是否在艾滋病发病机制中扩张或收缩，我们对54只SIV或SHIV感染的猕猴和48只未感染的对照进行了横断面研究。在急性感染动物、血浆病毒RNA不可检测的慢性感染动物、血浆病毒RNA可检测的慢性感染动物和死于AIDS的动物中检查单核细胞群的绝对数量。与未感染的对照组相比，在血浆病毒RNA可检测的急性感染动物和慢性感染动物中，CD 14（高）CD 16（低）和CD 14（低）CD 16（高）单核细胞的绝对数量显著升高。此外，感染队列中CD 14（高）CD 16（低）或CD 14（低）CD 16（高）单核细胞数量与血浆病毒载量之间存在显着的正相关性。这些数据显示了慢病毒感染期间血液单核细胞的动态变化，最显著的是CD 14（高）CD 16（低）单核细胞，其对疾病阶段具有特异性。J. Leukoc. 87：557-567; 2010.
Infection by HIV is associated with the expansion of monocytes expressing CD16 antigens, but the significance of this in HIV pathogenesis is largely unknown. In rhesus macaques, at least three subpopulations of blood monocytes were identified based on their expression of CD14 and CD16: CD14(high)CD16(-), CD14(high)CD16(low), and CD14(low)CD16(high). The phenotypes and functions of these subpopulations, including CD16(+) monocytes, were investigated in normal, uninfected rhesus macaques and macaques that were infected with SIV or chimeric SHIV. To assess whether these different monocyte subpopulations expand or contract in AIDS pathogenesis, we conducted a cross-sectional study of 54 SIV-or SHIV-infected macaques and 48 uninfected controls. The absolute numbers of monocyte populations were examined in acutely infected animals, chronically infected animals with no detectable plasma virus RNA, chronically infected animals with detectable plasma virus RNA, and animals that died with AIDS. The absolute numbers of CD14(high)CD16(low) and CD14(low)CD16(high) monocytes were elevated significantly in acutely infected animals and chronically infected animals with detectable plasma virus RNA compared with uninfected controls. Moreover, a significant, positive correlation was evident between the number of CD14(high)CD16(low) or CD14(low)CD16(high) monocytes and plasma viral load in the infected cohort. These data show the dynamic changes of blood monocytes, most notably, CD14(high)CD16(low) monocytes during lentiviral infection, which are specific to disease stage. J. Leukoc. Biol. 87: 557-567; 2010.