Quinoline and naphthalene derivatives from Saccharopolyspora sp. YIM M13568
Quinoline and naphthalene derivatives from Saccharopolyspora sp. YIM M13568
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DOI：
10.1038/ja.2016.142
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发表时间：
2016-11
期刊：
影响因子：
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通讯作者：
Mingwei Sun;Jinhuan Ou;Wenjun Li;Chunhua Lu
中科院分区：
文献类型：
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作者：
Mingwei Sun;Jinhuan Ou;Wenjun Li;Chunhua Lu
Quinoline, a well-known heterocyclic compound, itself has few applications; however, many of its derivatives are useful in diverse therapeutic applications, 1, 2 such as antimalarials (Quinine and Quinidine), antiviral (Saquinavir) and anticancer (Camptothecin, Irinotecan and Topotecan). 3 Over the past decades, the syntheses and the biological activities of quinoline-5, 8-diones have been reported. 2, 4, 5 In addition, various functionalized quinoline-5, 8-dione moieties are present in many classes of biologically active compounds, such as antitumor agents lavendamycim, 6 streptonigrin 7, 8 and styrylquinoline derivatives with perspective HIV integrase inhibitors. 9, 10 In the course of screening for bioactive metabolite from microbial sources, a strain Saccharopolyspora sp. YIM M13568 isolated from the mangrove rhizosphere was selected for (1) its excellent cytotoxic activity;(2) kinds of compounds with MWs in the range of 200− 300 amu possessing the UV chromophores at~ 350, 300, 260 and 220nm. In this study, we reported fermentation, isolation and structural elucidation of four quinoline alkaloids (1–4) and a new naphthalene derivative (5) from Saccharopolyspora sp. YIM M13568, and their cytotoxic activities.The strain YIM M13568, isolated from the mangrove rhizosphere samples, was identified as Saccharopolyspora sp. according to its 16 S rDNA sequence. The strain was inoculated on MP agar media (10 l) and cultured for 12 days at 28 C, and the fermented cultures were extracted with EtOAc-MeOH (85: 15, v/v) to obtain a crude extract. The crude extract was partitioned between EtOAc and H2O, and the EtOAc extract was further partitioned between 95% MeOH and petroleum ether to afford the MeOH extract (4.0 g). The MeOH extract was subjected to column chromatography over Sephadex LH-20, MPLC and finally purified by HPLC to afford 1–5 (Figure 1), respectively.
