Rapid (10-minute) stimulation of rat duodenal alkaline phosphatase activity by 1,25-dihydroxyvitamin D3.
Rapid (10-minute) stimulation of rat duodenal alkaline phosphatase activity by 1,25-dihydroxyvitamin D3.
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1,25-二羟基维生素 D3 快速（10 分钟）刺激大鼠十二指肠碱性磷酸酶活性。
作者：
L. Ben Nasr;J. Monet;P. A. Lucas
最近的报道表明，骨化三醇的作用比之前认为的要快得多。因此，一些活动可能不依赖于从头合成蛋白质。用精密显微定量技术研究了大鼠十二指肠绒毛刷状缘碱性磷酸酶(AP)对骨化三醇作用的时间过程，因为AP的活性依赖于动物体内的维生素D状态。这项技术可以在不破坏组织的情况下原位测定AP的活性。维生素D缺乏的雄性Wistar房颤大鼠ip骨化三醇后，AP出现双相反应，早期峰值出现在1h内(0.068+/-0.011比0.101+/-0.003微米3×10(3)，P<0.001)，第二次峰值出现在6~8h(0.088+/-0.005比0.172+/-0.003 IE/分钟3×10(3)，P<0.001)。在进一步的实验中，在给予骨化三醇或赋形剂(n=5对每个时间点)后0、10、30、45和60分钟观察对骨化三醇的早期反应。骨化三醇组AP活性在给药后10min明显高于赋形剂组(0.132+/-0.003比0.151+/-0.005 IE/min.microron 3×10(3)，P<0.02)，45min达高峰，此时AP活性与正常维生素D补充组相当(0.193+/-0.003比0.192+/-0.002 IE/min.microron(3)×10(3)，P>0.5)。这种反应的速度表明它不太可能依赖从头合成蛋白质。
Recent reports have suggested that the action of calcitriol is much more rapid than previously thought. It is thus possible that some actions do not depend on de novo protein synthesis. A precise microdensitometric technique has been used to characterize the time course of the intestinal brush border alkaline phosphatase (AP) response of rat duodenal villi to the administration of calcitriol as AP activity has been shown to be dependent on the vitamin D status of the animal. The technique enables AP activity to be determined in situ without tissue disruption. After ip administration of 200 ng calcitriol to vitamin D-deficient male Wistar AF rats, a biphasic AP response was observed with an early peak within 1 h (0.068 +/- 0.011 vs. 0.101 +/- 0.003 integrated extinction (IE) min.micron 3 X 10(3), P less than 0.05) and a second at between 6 and 8 h (0.088 +/- 0.005 vs. 0.172 +/- 0.003 IE/min.micron 3 X 10(3), P less than 0.001). In a further experiment, the early response to calcitriol was reexamined with observations at 0, 10, 30, 45, and 60 min after administration of either calcitriol or vehicle (n = 5 pairs per time point). AP activity was significantly increased in the calcitriol group compared with the vehicle-treated group as early as 10 min after administration (0.132 +/- 0.003 vs. 0.151 +/- 0.005 IE/min.micron 3 X 10(3), P less than 0.02) and reached a peak 45 min after administration at which time AP activity was equal to that found in normal vitamin D-replete animals (0.193 +/- 0.003 vs. 0.192 +/- 0.002 IE/min.micron (3) X 10(3), P greater than 0.5). The speed of this response indicates it to be unlikely to depend on de novo protein synthesis.
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1,25-二羟基维生素 D3 刺激小鸡十二指肠绒毛中的腺苷酸环化酶。
影响因子：
4.8
作者：
Long,RG;Bikle,DD;Munson,SJ
通讯作者：
Munson,SJ
维生素 D 状态和刷状缘膜囊泡：1,25-二羟基维生素 D3 诱导不稳定。
DOI：
10.1002/jbmr.5650010402
发表时间：
1986
期刊：
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research
影响因子：
--
作者：
Putkey,JA;Nemere,I;Norman,AW
通讯作者：
Norman,AW
高钙血症抑制 1,25-二羟基维生素 D3 在体外介导的正常雏鸡灌注十二指肠中钙转运的快速刺激作用。
影响因子：
4.8
作者：
Yoshimoto,Y;Nemere,I;Norman,AW
通讯作者：
Norman,AW
DOI：
10.1152/ajpgi.1986.250.3.g369
发表时间：
1986-03
期刊：
The American journal of physiology
影响因子：
--
作者：
D. B. Lee;M. Walling;N. Brautbar
通讯作者：
D. B. Lee;M. Walling;N. Brautbar
根据绒毛位置，十二指肠上皮细胞对 1,25-二羟基维生素 D3 的差异反应：钙摄取、钙结合蛋白和碱性磷酸酶活性的比较。
影响因子：
4.8
作者：
Bikle,DD;Zolock,DT;Munson,S
通讯作者：
Munson,S