Effects of glutamine supplementation on innate immune response in rats with gut-derived sepsis
Effects of glutamine supplementation on innate immune response in rats with gut-derived sepsis
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DOI：
10.1079/bjn20031069
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发表时间：
2004-03
影响因子：
3.6
通讯作者：
S. Yeh;Yu-Ni Lai;H. Shang;Ming-Tsan Lin;Wei‐Jao Chen
中科院分区：
文献类型：
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作者：
S. Yeh;Yu-Ni Lai;H. Shang;Ming-Tsan Lin;Wei‐Jao Chen
本研究探讨了脓毒症前富含谷氨酰胺（Gln）的饮食或脓毒症后含谷氨酰胺的全肠外营养（TPN），或两者兼而有之，对肠源性脓毒症大鼠的吞噬活性和血液淋巴细胞亚群的影响。将大鼠分配到对照组或四个实验组之一。对照组和第1、2组饲喂半纯化饲料;第3、4组用谷氨酰胺替代部分酪蛋白。喂养10 d后，采用盲肠结扎穿孔法（CLP）建立脓毒症模型，CLP后维持TPN 3 d。对照组、第1组和第3组采用常规TPN，第2组和第4组在TPN中添加谷氨酰胺。CLP或假手术后3d处死所有大鼠，检查其免疫反应。结果表明，与对照组相比，第3组和第4组腹腔巨噬细胞的吞噬活性增强，而第1组和第2组则无明显变化。第1组的CD 3+细胞比例显著低于对照组（P<0·05），而对照组和补充Gln组之间无差异。与对照组、3组和4组相比，第1组的CD 4+细胞比例显著降低（P<0.05）。这些结果表明，谷氨酰胺丰富的饮食CLP前显着增强腹腔巨噬细胞吞噬活性，保存CD 4+细胞和维持血液总T淋巴细胞在肠源性脓毒症。然而，盲肠结扎和穿刺后胃肠外谷氨酰胺给药对调节脓毒症大鼠的免疫应答没有有利的作用。
The present study examined the effect of glutamine (Gln)-enriched diets before sepsis or Gln-containing total parenteral nutrition (TPN) after sepsis, or both, on the phagocytic activity and blood lymphocyte subpopulation in rats with gut-derived sepsis. Rats were assigned to a control group or one of four experimental groups. The control group and groups 1 and 2 were fed a semipurified diet; groups 3 and 4 had part of casein replaced by Gln. After feeding the diets for 10d, sepsis was induced by caecal ligation and puncture (CLP); TPN was maintained for 3d after CLP. The control group and groups 1 and 3 were infused with conventional TPN and groups 2 and 4 were supplemented with Gln in the TPN solution. All rats were killed 3d after CLP or sham operation to examine their immune responses. The results showed that compared with the control group, the phagocytic activities of peritoneal macrophages were enhanced in groups 3 and 4, but not in groups 1 and 2. The proportion of CD3+ cells in group 1 was significantly lower (P<0·05) than that of the control group, whereas no differences were observed among the control and Gln-supplemented groups. The CD4+ cell proportion was significantly lower (P<0·05) in group 1 compared with the control group and groups 3 and 4. These findings suggest that Gln-enriched diets before CLP significantly enhanced peritoneal macrophage phagocytic activity, preserved CD4+ cells and maintained blood total T lymphocytes in gut-derived sepsis. However, parenteral Gln administration after caecal ligation and puncture had no favourable effects on modulating immune response in septic rats.