Cytotoxic and antioxidative activities of Plantago lagopus L. and characterization of its bioactive compounds
Cytotoxic and antioxidative activities of Plantago lagopus L. and characterization of its bioactive compounds
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DOI：
10.1016/j.fct.2012.01.019
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发表时间：
2012-05-01
影响因子：
4.3
通讯作者：
Saracoglu, Iclal
中科院分区：
文献类型：
--
作者：
Harput, U. Sebnem;Genc, Yasin;Saracoglu, Iclal
为了解车前属植物在传统医药中的应用，对车前属植物地上部分的化学成分进行了生物活性导向的分离和鉴定。基于MIT生长抑制试验，该提取物显示出与已知抗氧化剂3-叔丁基-4-羟基苯甲醚、抗坏血酸（维生素C）和槲皮素相当的对DPPH、一氧化氮（NO）和超氧（SO）自由基的强自由基清除作用，以及其对HEP-2（人喉表皮样癌）和RD（人横纹肌肉瘤）细胞系的细胞毒性活性。该植物的没食子酸当量总酚含量为79.94 mg/g干提取物。从活性最强的部位中分离得到苯乙醇苷类、毛蕊花苷和calceorioside A，这两种化合物对受试自由基具有较强的清除活性，对HEP-2具有细胞毒性。RD和MCF-7（人乳腺癌）细胞系。此外，在组织学分析中观察到凋亡细胞死亡。综上所述，这些发现表明毛蕊花苷和calceorioside A可用于预防癌症。(C)2012爱思唯尔有限公司保留所有权利。
Bioactivity guided isolation and characterization of phytoconstituents from the aerial parts of Plantago lagopus L were performed to give a new insight into the usage of Plantago species in traditional medicine. The extract showed strong radical scavenging effects against DPPH, nitric oxide (NO) and superoxide (SO) radicals comparable to that of known antioxidants 3-t-butyl-4-hydroxyanisole, ascorbic acid (vitamin C), and quercetin in addition to its cytotoxic activities against HEP-2 (human larynx epidermoid carcinoma) and RD (human rhabdomyosarcoma) cell lines based on MIT assay for growth inhibition. The gallic acid equivalent total phenolic content of the plant was found to be 79.94 mg/g dry extract. Phenylethanoid glycosides, verbascoside and calceorioside A were isolated from the most active fraction and both compounds showed strong radical scavenging activity against tested radicals and cytotoxicity against HEP-2. RD and MCF-7 (human breast adenocarcinoma) cell lines. In addition apoptotic cell death was observed in histological analysis. Taken together, these findings suggest that verbascoside and calceorioside A may be used in cancer prevention. (C) 2012 Elsevier Ltd. All rights reserved.