A novel stereotaxic approach to the hypothalamus for the use of push-pull perfusion cannula in Holstein calves
A novel stereotaxic approach to the hypothalamus for the use of push-pull perfusion cannula in Holstein calves
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DOI：
10.1016/j.jneumeth.2004.06.006
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发表时间：
2005-01-30
影响因子：
3
通讯作者：
Hodate, K
中科院分区：
文献类型：
--
作者：
Kasuya, E;Sakumoto, R;Hodate, K
为研究生长激素释放激素（GHRH）和生长抑素（SS）的分泌模式及其在生长激素（GH）分泌调节中的作用，建立了犊牛下丘脑灌流液的收集方法--推拉灌流法。使用牛的立体定位装置，根据脑室造影术将套管植入四头雄性小牛的下丘脑中。以10 min间隔收集推拉灌流液6 h，采用EIA和RIA分别测定灌流液中GHRH和SS浓度以及血浆GH浓度。根据第三脑室图像将套管植入下丘脑并维持1个月。GHRH和SS呈脉冲式分泌，清醒动物GHRH和SS的脉冲不规则。GHRH和SS的分泌与GH的分泌均无明显关系。因此，在本研究中，我们（1）建立了一个立体定位技术，接近下丘脑使用脑室造影小牛。（2）证明清醒犊牛GHRH和SS的分泌是脉动性的，但与GH谱无密切关系。该技术可用于研究牛下丘脑对垂体激素的调节作用。(C)2004 Elsevier B. V.保留所有权利。
To determine secretory patterns of growth hormone-releasing hormone (GHRH) and somatostatin (SS) and their roles in the regulation of growth hormone (GH) secretion, a method for collecting hypothalamic perfusates, a push-pull perfusion method was developed in calves. With the use of the stereotaxic apparatus for cattle, a cannula was implanted into the hypothalamus of four male calves based upon cerebral ventriculography. Push-pull perfusates were collected at 10 min intervals for 6 h and GHRH and SS concentrations in perfusates and plasma GH concentration were determined by EIAs and RIA, respectively. A cannula was implanted into the hypothalamus based on the image of the third ventricle and maintained for 1 month. GHRH and SS showed pulsatile secretion and the pulses for GHRH and SS were irregular in conscious animals. Neither GHRH nor SS secretion had a clear relationship with GH secretion. In the present study, we thus (1) established a stereotaxic technique for approaching the hypothalamus using cerebral ventriculography for calves. and (2) demonstrated that GHRH and SS secretion were pulsatile but not closely related to GH profile in conscious calves. The technique is useful for the study of the functions of the hypothalamus in the control of pituitary hormones in cattle. (C) 2004 Elsevier B.V. All rights reserved.