Comparison of structures and anticoagulant activities of fucosylated chondroitin sulfates from different sea cucumbers
Comparison of structures and anticoagulant activities of fucosylated chondroitin sulfates from different sea cucumbers
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DOI：
10.1016/j.carbpol.2010.08.040
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发表时间：
2011-01-10
影响因子：
11.2
通讯作者：
Chai, Wengang
中科院分区：
文献类型：
--
作者：
Chen, Shiguo;Xue, Changhu;Chai, Wengang
从印度-太平洋海参、西印度洋刺参、挪威海岸海参和西大西洋海参中分离得到岩藻糖化软骨素硫酸酯(FCS)。对FCS的详细序列，特别是它们的岩藻糖链进行了分析和比较。核磁共振氢谱(H-1)和碳谱(C-13)清晰地鉴定了4-0-单-2，3-0-二-和2-4-0-二硫化岩藻糖的3种不同的硫酸化模式。根据多糖的单糖组成和二维核磁共振波谱建立了硫酸软骨素骨架。对四种FCS的抗凝血活性进行了评价和比较，结果表明，它们抗凝血活性的差异可以归因于硫酸软骨素岩藻糖链上不同的硫酸盐化模式，而2，4-0-二硫化是抗凝活性的重要因素(C)2010爱思唯尔有限公司版权所有
Fucosylated chondroitin sulfates (fCSs) were isolated from four sea cucumbers Pearsonothuria graeffet (Indo-Pacific) Stichopus tremulus (Western Indian Ocean) Holothuria vagabunda (Norwegian coast) and Isostichopus badionotus (Western Atlantic) The detailed sequences of fCSs particularly their fucose branches were characterized and compared H-1 and C-13 NMR of the polysaccharides clearly identified three different sulfation patterns on the branched fucoses 4-0-mono- 2 3-0-di- and 2 4-0-di-sulfation variously distributed in the four fCSs The chondroitin sulfate backbones were established based on the monosaccharide composition of the polysaccharides and two-dimensional NMR of their oligosaccharide fragments Anticoagulant activities of the four fCSs were assessed and compared The results indicated that the difference in their anticoagulant activities can be attributed to the difference in sulfation pattern of the fucose branch of the chondroitin sulfate and 2 4-0-disulfation is important for anticoagulant activity (C) 2010 Elsevier Ltd All rights reserved