Naked eye detection of glutathione in living cells using rhodamine B-functionalized gold nanoparticles coupled with FRET
Naked eye detection of glutathione in living cells using rhodamine B-functionalized gold nanoparticles coupled with FRET
复制标题
使用罗丹明 B 功能化金纳米粒子结合 FRET 肉眼检测活细胞中的谷胱甘肽
DOI：
10.1016/j.dyepig.2011.06.016
复制
发表时间：
2012-01-01
影响因子：
4.5
通讯作者：
Cai, Jiye
中科院分区：
文献类型：
--
作者：
Cai, Huai-Hong;Wang, Hui;Cai, Jiye
In this study, functionalized gold nanoparticles, in combination with fluorescence resonance energy transfer (FRET), was utilized to visually detect glutathione (GSH) in aqueous samples and then demonstrated its applicability by estimating GSH level in living cells. We first modified the Au NPs with rhodamine B (RB), ON state of FRET, using the electrostatic interaction. In the presence of GSH, the competitive reaction between RB-functionalized nanoprobes (RB-Au NPs) and GSH caused the release of RB molecules from Au NP surface, OFF state of FRET, which was monitored simply by the naked eye. Fluorescence spectra and UV-vis absorption spectra were employed to investigate the spectroscopic characteristics of nanoprobes. Furthermore, the assay conditions, such as nanoprobe concentration, reaction time and the influence of interferent, were investigated. The visible color change of RB-Au NPs solution from dark blue to red in the presence of GSH was observed when the GSH concentration exceeded 10 mu M. This one-step naked eye detection depicted here successfully applied for the sensing of human hepatoma (HepG2) intracellular GSH level, showing the potential use in clinical nanodiagnosis. This method allows the rapid colorimetric detection of intracellular GSH without any preliminary treatment and specific instruments. (C) 2011 Elsevier Ltd. All rights reserved.
