Evaluation of the avoximeter: Precision, long-term stability, linearity and use without heparin
Evaluation of the avoximeter: Precision, long-term stability, linearity and use without heparin
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DOI：
10.1023/a:1007308616686
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发表时间：
1997-05-01
期刊：
影响因子：
--
通讯作者：
Martinez, A
中科院分区：
文献类型：
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作者：
Bailey, SR;Russell, EL;Martinez, A
目标。由于 AVOXimeter 使用一次性比色皿，直接在全血中进行测量，无需首先溶血样本，因此它不需要传统血氧计所需的维护和保养，它比传统血氧计运行速度更快，而且价格更便宜。因此，本研究的目的是（1）评估AVOXimeter测量总血红蛋白浓度和氧合血红蛋白饱和度的精度和线性度； (2) 评估其长期稳定性，从而评估所需的重新校准间隔； (3) 确定是否可以在没有抗凝剂的情况下进行测量； (4)评估在一次性比色皿中储存血液样本的可行性。方法。将测试仪器的测量结果与传统血氧计或标准化血红蛋白溶液的测量结果进行比较。还收集了含有或不含肝素的血样，以确定是否需要凝血。结果。我们的测试证实了总血红蛋白的指定精度为 0.3 g/dl，氧合血红蛋白的指定精度为 0.5%。结果还表明，与传统血氧计相比，这些测量结果呈线性，并且与总血红蛋白 0.45 g/dl 和氧合血红蛋白 1% 的指定精度一致。每周检查对照溶液表明仪器可以保持校准一年或更长时间。尽管用肝素处理注射器会导致稀释误差，但避免稀释时，肝素不会影响测量。当将血样放入一次性比色皿中并以 1 分钟的间隔重复读取 20 分钟时，读数明显偏离原始值。这种漂移发生得非常缓慢，以至于比色皿装满后第一分钟和第二分钟所获得的读数与原始读数的偏差在 1% 或 2% 以内。结论。根据我们的经验，测试仪器简单且易于操作。它满足精度和准确度的规格，测量结果高度线性，并且保持稳定校准一年。一旦抽血后，比色皿就充满了，因此不需要抗凝。然而，比色皿应在比色皿充满 1 密尔的情况下读取。
Objectives. Because the AVOXimeter uses disposable cuvettes and makes its measurements directly in whole blood without first hemolyzing the sample, it does not need the care and maintenance that conventional co-oximeters require, it operates faster than conventional co-oximeters, and it is less expensive. Therefore, the objectives of this study were (1) to evaluate the precision and linearity of the AVOXimeter's measurements of total hemoglobin concentration and oxyhemoglobin saturation; (2) to assess its long-term stability and thus the required interval for recalibration; (3) to determine whether measurements can be made without anticoagulants; and (4) to assess the feasibility of storing blood samples in the disposable cuvettes. Methods. Measurements made by the test instrument were compared with those of conventional co-oximeters or with standardized hemoglobin solutions. Blood samples were also collected with and without heparin to determine whether and-coagulation is necessary. Results. Our tests confirmed the specified precision of 0.3 g/dl for total hemoglobin and 0.5% for oxyhemoglobin. The results also showed that these measurements were linear when compared with a conventional co-oximeter, and they were consistent with the specified accuracy of 0.45 g/dl for total hemoglobin and 1% for oxyhemoglobin. Weekly checks with control solutions showed that the instrument holds its calibration for a year or more. Although treating syringes with heparin caused dilution errors, heparin did not affect the measurements when dilution was avoided. When blood samples were placed in disposable cuvettes and read repeatedly at 1-min intervals for 20 min, the readings drifted appreciably away from the original value. This drift occurred so slowly that readings taken at the first and second minute after the cuvette was filled were within 1 or 2% of the original reading. Conclusions. In our experience, the test instrument was simple and easy to operate. It met the specifications for precision and accuracy, its measurements were highly linear, and it maintained a stable calibration for one year. Ii the cuvettes are filled as soon as blood is drawn, anticoagulation is unnecessary. However, the cuvettes should be read with 1 mill of filling the cuvette.