Yeast identification: reassessment of assimilation tests as sole universal identifiers.
Yeast identification: reassessment of assimilation tests as sole universal identifiers.
复制标题
酵母鉴定：重新评估同化测试作为唯一的通用标识符。
DOI：
10.1111/j.1472-765x.2011.03130.x
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发表时间：
2011
影响因子：
2.4
通讯作者：
Spencer J
中科院分区：
文献类型：
--
作者：
Spencer J
目的：评估同化试验是否仍然是一个有效的方法，酵母菌的鉴定，当应用于广泛的环境和腐败isolations.Methods和结果：七十一酵母菌株分离自软饮料厂。这些都是通过同化试验和D1/D2 rDNA测序鉴定的。与测序相比，同化测试鉴定（MicroLog™）的正确率为18.3%，属内的正确率为14.1%，错误鉴定率为67.6%。后者的大部分可以归因于新报道的酵母species.Conclusions的上升：同化试验本身是不可靠的，作为一个通用的酵母菌鉴定的手段，因为许多新的物种，菌株的变异性和同化配置文件的一致性增加。同化试验仍然有一个有用的作用，在鉴定常见的物种，如大多数的临床分离株。意义和影响的研究：这是可能的，根据这些结果，许多酵母鉴定旧文献报道是不正确的。这强调了在目前和今后的出版物中准确识别的迫切需要。
Aims:To assess whether assimilation tests in isolation remain a valid method of identification of yeasts, when applied to a wide range of environmental and spoilage isolates.Methods and Results:Seventy‐one yeast strains were isolated from a soft drinks factory. These were identified using assimilation tests and by D1/D2 rDNA sequencing. When compared to sequencing, assimilation test identifications (MicroLog™) were 18·3% correct, a further 14·1% correct within the genus and 67·6% were incorrectly identified. The majority of the latter could be attributed to the rise in newly reported yeast species.Conclusions:Assimilation tests alone are unreliable as a universal means of yeast identification, because of numerous new species, variability of strains and increasing coincidence of assimilation profiles. Assimilation tests still have a useful role in the identification of common species, such as the majority of clinical isolates.Significance and Impact of the Study:It is probable, based on these results, that many yeast identifications reported in older literature are incorrect. This emphasizes the crucial need for accurate identification in present and future publications.