Super-resolution microscopy using normal flow decoding and geometric constraints
Super-resolution microscopy using normal flow decoding and geometric constraints
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DOI：
10.1046/j.1365-2818.2001.00950.x
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发表时间：
2001-11-01
影响因子：
2
通讯作者：
Danuser, G
中科院分区：
文献类型：
--
作者：
Danuser, G
关于观察到的场景的先验知识提供了恢复超出成像系统的带通的频率（超分辨率）的关键。结合显微镜的两个超分辨率机制已被主要报道：频谱的解析延拓和约束图像反卷积。本文介绍了一种替代方法的超分辨率。先验知识通过场景的几何和动态模型来施加。我们说明了我们的概念的基础上的立体重建的微量移液器移动接近一个固定的目标对象。关于移液管的形状和移动的信息被并入重建算法中。该算法在微型机器人环境中进行了测试，其中移液器吸头在到目标的亚瑞利距离处被跟踪。基于跟踪结果，机器视觉模块控制微观物体的操作，例如乳胶珠或金刚石单晶。在本文的理论部分，我们证明了知识的形式“移液器之间移动的两个连续帧的电影”必须导致分辨率的两倍增加。我们使用图像序列的正常流来从运动证据解码位置测量。在实践中，获得了3和5之间的超分辨率因子。额外的增益源自施加在移液管轴的立体重建上的几何约束。
Prior knowledge about the observed scene provides the key to restoration of frequencies beyond the bandpass of an imaging system (super-resolution). In conjunction with microscopy two super-resolution mechanisms have been mainly reported: analytic continuation of the frequency spectrum, and constrained image deconvolution. This paper describes an alternative approach to super-resolution. Prior knowledge is imposed through geometric and dynamic models of the scene. We illustrate our concept based on the stereo reconstruction of a micropipette moving in close proximity to a stationary target object. Information about the shape and the movement of the pipette is incorporated into the reconstruction algorithm. The algorithm was tested in a microrobot environment, where the pipette tip was tracked at sub-Rayleigh distances to the target. Based on the tracking results, a machine vision module controlled the manipulation of microscopic objects, e.g. latex beads or diamond mono-crystals. In the theoretical part of this paper we prove that knowledge of the form 'the pipette has moved between two consecutive frames of the movie' must result in a twofold increase in resolution. We used the normal flow of an image sequence to decode positional measures from motion evidence. In practice, super-resolution factors between 3 and 5 were obtained, The additional gain originates from the geometric constraints that were imposed upon the stereo reconstruction of the pipette axis.