The murine CD99-related molecule CD99-like 2 (CD99L2) is an adhesion molecule involved in the inflammatory response
The murine CD99-related molecule CD99-like 2 (CD99L2) is an adhesion molecule involved in the inflammatory response
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DOI：
10.1080/15419060701755966
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发表时间：
2007-09-01
影响因子：
--
通讯作者：
Muller, William A.
中科院分区：
文献类型：
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作者：
Schenkel, Alan R.;Dufour, Eric M.;Muller, William A.
CD99是一种存在于白细胞表面的糖蛋白，集中在内皮细胞的边缘，在白细胞通过内皮细胞迁移到炎症部位起主要作用，在胸腺细胞的发育中也有其他作用。人类和小鼠的基因组只编码两种与CD99相关的蛋白质。其中之一，XGA，是一种红细胞表面抗原。另一个是CD99样蛋白2(CD99L2)，其功能尚不清楚。我们克隆了小鼠CD99L2，并用从转基因细胞中分离的CD99L2制备了特异性抗体。与人CD99相似，CD99L2在细胞交界处以及小鼠白细胞和血管内皮细胞上均有原位表达。将CD99L2基因导入L细胞成纤维细胞，使其能够以二价阳离子依赖的亲和性方式相互黏附。抗CD99L2抗体在体内阻止中性粒细胞和单核细胞进入炎症部位。
CD99, a glycoprotein found on the surfaces of leukocytes and concentrated at the borders of endothelial cells, plays a major role in the migration of leukocytes across endothelial cells into sites of inflammation, and has other roles in thymocyte development. The human and mouse genomes encode only two proteins related to CD99. One of these, XGA, is a red blood cell surface antigen. The function of the other, CD99-like 2 (CD99L2), is not known. We cloned mouse CD99L2 and used CD99L2 isolated from transfected cells to raise specific antibodies. Similar to human CD99, CD99L2 was expressed at the borders between transfected cells as well as on mouse leukocytes and vascular endothelial cells in situ. Transfection of L cell fibroblasts with CD99L2 imparted to them the ability to adhere to each other in a divalent cation-dependent, homophilic manner. Anti-CD99L2 antibody blocked influx of neutrophils and monocytes into a site of inflammation in vivo.