Identification of a lineage negative cell population in bovine peripheral blood with the ability to mount a strong type I interferon response
Identification of a lineage negative cell population in bovine peripheral blood with the ability to mount a strong type I interferon response
复制标题
DOI：
10.1016/j.dci.2011.05.002
复制
发表时间：
2012-02-01
影响因子：
2.9
通讯作者：
Werling, Dirk
中科院分区：
文献类型：
--
作者：
Gibson, Amanda;Miah, Suhel;Werling, Dirk
谱系阴性树突状细胞，或天然干扰素产生细胞（NIPC），也被称为浆细胞样树突状细胞（pDC），构成一小群白细胞，在响应某些危险信号时分泌高水平的I型干扰素（IFN α / β）。在这里，我们为鉴定迄今为止未表征的循环牛pDC样细胞提供了初步数据。从PBMC中分离到一个谱系阴性细胞群（LIN-细胞），其特征与其他物种的pDC相似。分离的LIN-细胞呈淋巴样形态，核呈半新月状，内质网分布广泛，具有高尔基网状结构；指示pDC。此外，LIN-细胞的表型分析表明它们与其他牛DC亚群不同；同时表达淋巴细胞和髓细胞表面标记物。LIN-细胞不表达谱系特异性标志物，但为MHC II类+、CD45RO（+）、CD80/86（+）、CD6（+）、WC1（+）、CD26(+)，并表达骨髓标志物CD205、CD172a和CD11a。与pDC一致，LIN-细胞表达TLR7 mRNA转录物；而静息状态下不表达TLR8或TLR9。在功能上，LIN-细胞，而不是PBMC，单核细胞和单核细胞衍生的DC产生大量的IFN α / β，以响应不同的CpG寡核苷酸。综上所述，我们目前的数据表明，一个丰富的牛LIN-细胞循环群体具有独特的能力，能够产生IFN α / β，以响应CpG寡核苷酸，因此这个群体可能含有与牛pDC功能相当的功能。(C) 2011 Elsevier Ltd.版权所有。
Lineage negative dendritic cells, or natural interferon-producing cells (NIPC), also referred to as plasmacytoid dendritic cells (pDC) constitute a small population of leukocytes secreting high levels of type I interferon (IFN alpha/beta) in response to certain danger signals. Here, we provide initial data towards the identification of so far uncharacterised circulating bovine pDC like cells. A lineage negative cell population (LIN- cells) was isolated from PBMC which showed characteristics similar to that of pDC in other species. Isolated LIN- cells presented lymphoid morphology with a semi-crescent nucleus, extensive ER and Golgi network; indicative of pDC. In addition phenotypic analysis of LIN- cells described them as distinct from other bovine DC subsets; expressing both lymphoid and myeloid surface markers. LIN- cells did not express lineage specific markers, but were MHC class II+, CD45RO(+), CD80/86(+), CD6(+), WC1(+), CD26(+) and expressed the myeloid markers CD205, CD172a and CD11a. In keeping with pDC, LIN- cells express TLR7 mRNA transcripts; however, in a resting state do not express TLR8 or TLR9. Functionally, LIN- cells, but not PBMC, monocytes and monocyte derived DC produce large amounts of IFN alpha/beta in response to different CpG oligonucleotides. Taken together, we present data suggesting that an enriched circulating population of bovine LIN- cells are uniquely capable of producing IFN alpha/beta in response to CpG oligonucleotides and thus this population likely contain the functional equivalent of bovine pDC. (C) 2011 Elsevier Ltd. All rights reserved.