Nucleolar localization of the microtubule-associated protein tau in neuroblastomas using sense and anti-sense transfection strategies.
Nucleolar localization of the microtubule-associated protein tau in neuroblastomas using sense and anti-sense transfection strategies.
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DOI：
10.1002/(sici)1097-0169(1997)38:1
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发表时间：
1997
影响因子：
--
通讯作者：
V. Thurston;P. Peña;R. Pestell;L. Binder
中科院分区：
文献类型：
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作者：
V. Thurston;P. Peña;R. Pestell;L. Binder
Tau-1单克隆抗体定位于间期细胞的核仁和培养的人细胞中的近端着丝粒染色体的核仁组织区（NORs）。在CG神经母细胞瘤细胞中发现了推定的核仁和NOR tau，其含有核仁tau和少量胞质tau。为了进一步确定tau在这些细胞的核仁中的存在，使用正义和反义转染策略。CG神经母细胞瘤细胞用tau正义cDNA转染并用Tau-1免疫染色。细胞质Tau-1染色在含有非常少的内源性细胞质tau的CG细胞中大大增加。在某些CG细胞中核仁Tau-1染色也增加，表明转染细胞亚组中核仁tau增加。CG细胞也用tau反义cDNA转染，其消除了核仁中的Tau-1染色。这些结果有助于越来越多的证据将tau定义为在灵长类细胞的细胞质和核仁中发现的多功能蛋白质。
The Tau-1 monoclonal antibody was localized to the nucleolus of interphase cells and the nucleolar organizing regions (NORs) of acrocentric chromosomes in cultured human cells. Putative nucleolar and NOR tau was found in CG neuroblastoma cells which contain nucleolar tau and little cytoplasmic tau. To further establish the presence of tau in the nucleolus of these cells, sense and anti-sense transfection strategies were used. CG neuroblastoma cells were transfected with tau sense cDNA and immunostained with Tau-1. Cytoplasmic Tau-1 staining was greatly increased in CG cells which contain very little endogenous cytoplasmic tau. Nucleolar Tau-1 staining was also increased in certain CG cells indicating an increase in nucleolar tau in a subset of transfected cells. CG cells were also transfected with tau anti-sense cDNA which abolished Tau-1 staining in the nucleolus. These results contribute to a growing body of evidence defining tau as a multifunctional protein found in both the cytoplasm and nucleoli of primate cells.