Visualization of cardiovascular development, physiology and disease at the single-cell level: Opportunities and future challenges
Visualization of cardiovascular development, physiology and disease at the single-cell level: Opportunities and future challenges
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DOI：
10.1016/j.yjmcc.2020.03.005
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发表时间：
2020-05-01
影响因子：
5
通讯作者：
Pu Jun
中科院分区：
文献类型：
--
作者：
Chen Yifan;Yang Fan;Pu Jun
单细胞RNA测序（scRNA-seq）是一种在单细胞水平上进行转录组测序的方法，最近已成为生物医学研究领域的革命性技术。与传统的批量基因表达谱分析相比，scRNA-seq解决了单个细胞之间的生物学差异，并能够识别容易被忽视的稀有细胞群。本文就scRNA-seq方法的研究进展进行综述，并对其在心血管疾病研究中的应用进行展望。
Single-cell RNA sequencing (scRNA-seq), a method of transcriptome sequencing at the single-cell level, has recently emerged as a revolutionary technology in the field of biomedical research. Compared to conventional gene expression profiling in bulk, scRNA-seq resolves biological differences among individual cells and enables the identification of rare cell populations that are easily overlooked. This review introduces the method of scRNA-seq, summarizes its applications in the field of cardiovascular disease research, and discusses existing limitations and prospects for future applications.