The level of C/EBP protein is critical for cell migration during Drosophila oogenesis and is tightly controlled by regulated degradation.
The level of C/EBP protein is critical for cell migration during Drosophila oogenesis and is tightly controlled by regulated degradation.
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DOI：
10.1016/s1097-2765(05)00008-0
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发表时间：
2000-07
期刊：
影响因子：
16
通讯作者：
Pernille Rørth;Kornelia Szabo;Gemma Texido
中科院分区：
文献类型：
--
作者：
Pernille Rørth;Kornelia Szabo;Gemma Texido
C/EBP转录因子Slbo是果蝇发生过程中边界细胞迁移所必需的。出乎意料的是，我们发现在边界细胞中既不能容忍Slbo活性的增加也不能容忍Slbo活性的减少。正确的蛋白水平在一定程度上是由细胞类型特异性调节的Slbo蛋白周转来保证的。通过基因筛选，我们确定了两个参与这一调控的基因。Ubp64泛素水解酶作为Slbo蛋白的稳定剂。一种新的基因，tribles，是体内激素的负调节因子。Tribbles通过特异性靶向Slbo通过泛素化快速降解而起作用。
The C/EBP transcription factor, Slbo, is required for migration of border cells duringDrosophilaoogenesis. Unexpectedly, we find that neither increase nor decrease of Slbo activity is tolerated in border cells. Correct protein level is in part ensured by cell type–specific regulated turnover of Slbo protein. Through genetic screening, we identify two genes that are involved in this regulation. The Ubp64 ubiquitin hydrolase acts as a stabilizer of Slbo protein. A novel gene,tribbles, is a negative regulator ofslboin vivo. Tribbles acts by specifically targeting Slbo for rapid degradation via ubiquitination.