FUSION OF DIPALMITOYLPHOSPHATIDYLCHOLINE VESICLES AT 4-DEGREES-C
FUSION OF DIPALMITOYLPHOSPHATIDYLCHOLINE VESICLES AT 4-DEGREES-C
复制标题
DOI：
10.1021/bi00260a032
复制
发表时间：
1982-01-01
期刊：
影响因子：
2.9
通讯作者：
THOMPSON, TE
中科院分区：
文献类型：
--
作者：
WONG, M;ANTHONY, FH;THOMPSON, TE
当在4 ℃下孵育时，超声处理的二棕榈酰磷脂酰胆碱小泡。C和高浓度完全融合到约700埃的囊泡中。直径，并且这些进一步融合到约950埃。3 - 4 wk后出现直径囊泡。第950章.直径的囊泡是球形的、均匀的、大部分是单层的，具有约10倍于小囊泡的内部水空间，并且稳定至少6个月。950-. ANG。通过琼脂糖凝胶色谱法、冷冻断裂EM、捕获体积测量、差示扫描量热法和二苯基己三烯荧光偏振来表征囊泡。
Small sonicated dipalmitoylphosphatidylcholine vesicles when incubated at 4.degree. C and high concentrations fuse completely to vesicles about 700-.ANG. diameter in 7 days, and these further fuse to about 950 .ANG. diameter vesicles after 3-4 wk. The 950 .ANG. diameter vesicles are spherical, homogeneous, mostly unilamellar, have an internal aqueous space about 10 times that of small vesicles, and are stable for at least 6 mo. The 950-.ANG. vesicles are characterized by agarose gel chromatography, freeze-fracture EM, trapped volume measurements, differential scanning calorimetry, and diphenylhexatriene fluorescence polarization.