Microbial-derived biosensors for monitoring environmental contaminants: Recent advances and future outlook
Microbial-derived biosensors for monitoring environmental contaminants: Recent advances and future outlook
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DOI：
10.1016/j.psep.2019.01.032
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发表时间：
2019-04-01
影响因子：
7.8
通讯作者：
Iqbal, Hafiz M. N.
中科院分区：
文献类型：
--
作者：
Bilal, Muhammad;Iqbal, Hafiz M. N.
活细胞已经进化到能够感知各种环境刺激，从而为设计生物传感设备提供了一个新的平台。由于易于遗传修饰和保存，微生物作为理想的宿主候选者获得了特殊的地位。此外，微生物衍生的生物传感器易于使用、便宜、紧凑和便携。独特的结构和功能特征使其特别适合检测和监测一系列与环境有关的污染物。调控基因的操作可以提升生物传感器的一系列特性，例如灵敏度、选择性和靶范围。这种基于遗传微生物的生物传感器的当代使用导致了具有用于监测环境污染的前所未有的潜力的生物传感器的制造。本文综述了近年来微生物生物传感器在环境污染物监测方面的研究进展。重点介绍了基因工程微生物传感器的改进及其在环境污染物监测中的应用。还强调了包括技术和社会在内的关键挑战，以破译微生物衍生生物传感器的真正前景，使其得到广泛采用和利用。最后，总结了工作的结论和未来的观点，以突出微生物衍生的生物传感器的价值，它可以实时，快速和独特的方式从更广泛的光谱中检测各种污染物或有毒化合物，具有高灵敏度和出色的选择性。(C)2019化学工程师学会。Elsevier B.V.出版，保留所有权利。
Living cells have evolved to sense various environmental stimuli, thus presenting a novel platform to engineer bio-sensing devices. Owing to the ease of genetic modification and preservation, microorganisms have gained a special place as ideal host candidates. Moreover, microbial-derived biosensors are easy to use, inexpensive, compact, and portable. The unique structural and functional features rendering them exceptionally suitable for detection and monitoring of an array of environmentally-related pollutants. The manipulation of regulatory genes could upgrade a range of characteristic features of a biosensor such as sensitivity, selectivity and, target range. Contemporary uses of such genetically-microbe-based biosensors led to the fabrication of biosensors with unprecedented potentialities for monitoring environmental pollution. Herein, we reviewed the current advances in microbial-derived biosensors for monitoring environmentally-related contaminants. A particular emphasis is given to the improvement of genetically-engineered microbial biosensors and their application for environmental pollutants monitoring with up-to-date examples. Critical challenges including technical and societal are also highlighted to decipher the true perspective of microbial-derived biosensors for their widespread adoption and exploitation. Towards the end, the work is wrapped up with concluding remarks and future viewpoints to highlight the value of the microbial-derived biosensors, which could detect various pollutants or toxic compounds from a broader spectrum in the real-time, rapid and unique way with high sensitivity, and outstanding selectivity. (C) 2019 Institution of Chemical Engineers. Published by Elsevier B.V. All rights reserved.