GLUTATHIONE S-TRANSFERASES IN LIVER METASTASES OF COLORECTAL-CANCER - A COMPARISON WITH NORMAL LIVER AND PRIMARY CARCINOMAS
GLUTATHIONE S-TRANSFERASES IN LIVER METASTASES OF COLORECTAL-CANCER - A COMPARISON WITH NORMAL LIVER AND PRIMARY CARCINOMAS
复制标题
DOI：
10.1093/carcin/15.10.2149
复制
发表时间：
1994-10-01
期刊：
影响因子：
4.7
通讯作者：
VERSPAGET, HW
中科院分区：
文献类型：
--
作者：
MULDER, TPJ;ROELOFS, HMJ;VERSPAGET, HW
在10对配对的结直肠癌患者的正常肝和肝转移中测定谷胱甘肽S-转移酶活性和谷胱甘肽S-转移酶-α、-mu和-pi水平。为了比较，分析了6对配对的结直肠癌和正常粘膜。与匹配的正常肝组织相比，所有转移瘤的谷胱甘肽S-转移酶活性均较低（分别为224 +/- 21和900 +/- 95 nmol/min.mg蛋白，P < 0.001）。原发性肿瘤和正常结直肠组织中的平均活性分别为176 +/- 22和150 +/- 13 nmol/min.mg蛋白。当通过免疫印迹技术分析时，每个转移瘤含有比周围正常肝脏更少的谷胱甘肽S-转移酶-α（平均值分别为3.3 +/- 0.8和21.8 +/- 1.8 μ g/mg蛋白，P < 0.001）。在所有原发性肿瘤和正常结肠粘膜中均检测不到谷胱甘肽S-转移酶-α。仅在两名肝转移患者和两名原发性结直肠癌患者中检测到谷胱甘肽S-转移酶-mu。所有转移瘤均含有比周围正常肝组织更多的谷胱甘肽S-转移酶-pi（分别为3.7 ± 0.5和0.4 ± 0.1 μ g/mg蛋白，P < 0.001）。转移瘤中的值与原发性结肠肿瘤中的值非常相似（正常粘膜2.3 +/- 0.3和肿瘤3.3 +/- 0.7 μ g/mg蛋白）。转移瘤的免疫组织化学研究显示，谷胱甘肽S-转移酶-α不位于恶性细胞中，而仅位于肉眼看来是纯转移组织的肝细胞中。谷胱甘肽S-转移酶-pi染色显示肿瘤细胞通常呈阳性，除胆管上皮外，肝细胞仅呈微弱染色。它的结论是，肝转移结直肠癌有非常相似的谷胱甘肽S-转移酶的活性和组成相比，与原发性肿瘤。
Glutathione S-transferase activity and levels of glutathione S-transferases-alpha, -mu and -pi were determined in 10 matched pairs of normal liver and liver metastasis from patients with colorectal cancer. For comparison, six matched pairs of colorectal cancer and normal mucosa were analysed. All metastases had a lower glutathione S-transferase activity when compared to the matched normal liver tissue (224 +/- 21 versus 900 +/- 95 nmol/min.mg protein respectively, P < 0.001). Mean activities in primary tumours and normal colorectal tissue were 176 +/- 22 and 150 +/- 13 nmol/min.mg protein respectively. When analysed by immunoblot techniques, each metastasis contained less glutathione S-transferase-alpha than the surrounding normal liver (mean values 3.3 +/- 0.8 versus 21.8 +/- 1.8 mu g/mg protein respectively, P < 0.001). Glutathione S-transferase-alpha was undetectable in all primary tumours and normal colonic mucosa. Glutathione S-transferase-mu was detected in only two patients with liver metastases and in two patients with primary colorectal canter. All metastases contained more glutathione S-transferase-pi than the surrounding normal liver tissue (3.7 +/- 0.5 versus 0.4 +/- 0.1 mu g/mg protein respectively, P < 0.001). The values in the metastases were very similar to those in the primary colonic tumours (normal mucosa 2.3 +/- 0.3 and tumours 3.3 +/- 0.7 mu g/mg protein). Immunohistochemical investigation of the metastases revealed that glutathione S-transferase-alpha is not located in the malignant cells, but only in hepatocytes in what macroscopically seemed to be pure metastatic tissue. Staining for glutathione S-transferase-pi reveals generally positive tumour cells and, except for the biliary epithelium, only faint staining of the hepatocytes. It is concluded that liver metastases of colorectal carcinomas have very similar glutathione S-transferase enzyme activities and composition as compared,vith primary tumours.