The Insertion Mechanism of a Living Cell Determined by the Stress Segmentation Effect of the Cell Membrane during the Tip-Cell Interaction
The Insertion Mechanism of a Living Cell Determined by the Stress Segmentation Effect of the Cell Membrane during the Tip-Cell Interaction
复制标题
尖端-细胞相互作用过程中细胞膜的应力分割效应决定活细胞的插入机制
DOI：
10.1002/smll.201703868
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发表时间：
2018-05-29
期刊：
影响因子：
13.3
通讯作者：
Peng, Bei
中科院分区：
文献类型：
--
作者：
Fan, Na;Jiang, Hai;Peng, Bei
原子力显微镜探针被证明是测量和操纵单个细胞的有力工具，为药物/蛋白质的可控递送提供了潜在的应用。然而，所测得的插入效率在20%到80%之间变化很大，在某些情况下，无论施加多大的力，纳米针尖都无法穿透细胞膜。因此，在该技术进入实际应用之前，必须彻底研究针尖-细胞相互作用期间活细胞的插入机制。在这项工作中，建立了一个多结构的细胞模型来研究尖端-膜相互作用。模拟结果表明，细胞膜的应力可以通过应力分割点S分为两个阶段。点S后，细胞膜的应力略有增加，大部分压痕力分配给细胞骨架。这种现象被称为“细胞膜的应力分割效应”，证实了基于实验研究的假设。此外，根据实验和数值研究，应力分割效应的假设也解释了为什么修饰细胞膜或使用人造尖锐的纳米尖端可以提高插入效率。
Atomic force microscopy probes are proved to be powerful tools to measure and manipulate the individual cell, providing potential applications for the controlled drug/protein delivery. However, the measured insertion efficiency varies dramatically from 20 to 80%, in some cases, the nanotip can never penetrate the cell membrane no matter how much force is applied to it. Thus, the insertion mechanism of a living cell during the tip-cell interaction must be thoroughly investigated before this technology comes into practical applications. In this work, a multistructural cell model is established to study the tip-membrane interaction. The simulation results show that the stress of the cell membrane can be divided into two stages by the stress segmentation point S. After point S, the stress of the cell membrane increases slightly and most of the indentation force is allocated to the cytoskeleton. This phenomenon is called "stress segmentation effect of the cell membrane," which confirms the hypothesis based on the experimental studies. Moreover, according to the experimental and numerical studies, the hypothesis of the stress segmentation effect also explains the reason that modifying the cell membrane or using the manmade sharpened nanotip can increase the insertion efficiency.