A systematic study of low-resolution recognition in protein-protein complexes
A systematic study of low-resolution recognition in protein-protein complexes
复制标题
DOI：
10.1073/pnas.96.15.8477
复制
发表时间：
1999-07-20
影响因子：
11.1
通讯作者：
Lam, CF
中科院分区：
文献类型：
--
作者：
Vakser, IA;Matar, OG;Lam, CF
利用475个共结晶蛋白-蛋白复合物的非冗余数据库研究低分辨率识别，这在早期的少量蛋白质对接实验中有报道。对接程序GRAMM用于删除原子大小的结构细节，系统对接得到的分子图像。结果表明，数据库中52%的配合物存在低分辨率识别，113个配合物中有76%的配合物存在低分辨率识别，界面面积为bb0 4000埃(2)。本研究中使用的对接和分析工具的局限性表明，具有低分辨率识别的配合物的实际数量更高。然而，研究结果已经在大范围内证明了低分辨率识别的存在。
A comprehensive nonredundant database of 475 cocrystallized protein-protein complexes was used to study low-resolution recognition, which was reported in earlier docking experiments with a small number of proteins. The docking program GRAMM was used to delete the atom-size structural details and systematically dock the resulting molecular images. The results reveal the existence of the low-resolution recognition in 52% of all complexes in the database and in 76% of the 113 complexes with an interface area > 4,000 Angstrom(2). Limitations of the docking and analysis tools used in this study suggest that the actual number of complexes with the low-resolution recognition is higher. However, the results already prove the existence of the low-resolution recognition on a broad scale.